| |  Online-Ressource |
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| Verfasst von: | Kingreen, Tim [VerfasserIn]  |
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| | Kewitz-Hempel, Stefanie [VerfasserIn] |
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| | Rohde, Christian [VerfasserIn] |
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| | Hause, Gerd [VerfasserIn] |
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| | Sunderkötter, Cord [VerfasserIn] |
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| | Gerloff, Dennis [VerfasserIn] |
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| Titel: | Extracellular vesicles from highly invasive melanoma subpopulations increase the invasive capacity of less invasive melanoma cells through mir-1246-mediated inhibition of CCNG2 |
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| Verf.angabe: | Tim Kingreen, Stefanie Kewitz-Hempel, Christian Rohde, Gerd Hause, Cord Sunderkötter and Dennis Gerloff |
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| E-Jahr: | 2024 |
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| Jahr: | 16 September 2024 |
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| Umfang: | 13 S. |
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| Illustrationen: | Illustrationen, Diagramme |
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| Fussnoten: | Gesehen am 18.02.2025 |
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| Titel Quelle: | Enthalten in: Cell communication and signaling |
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| Ort Quelle: | London : Biomed Central, 2003 |
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| Jahr Quelle: | 2024 |
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| Band/Heft Quelle: | 22(2024), 1, Artikel-ID 442, Seite 1-13 |
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| ISSN Quelle: | 1478-811X |
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| Abstract: | Invasive growth is a critical process in tumor progression, requiring the activation of various molecular processes in tumor cells at the invasive front. Intercellular communication between heterogeneous tumor cells enhances cellular activation and adaptation to specific microenvironments. One mechanism of intercellular communication is the delivery of miRNAs through tumor cell-derived extracellular vesicles (EVs). In this context we have observed that conditioned media from a highly invasive cell subpopulation (BLM-HI) enhances the invasive capacity of the parental cell line (BLM). Therefore, we hypothesized that this complex change of cellular behavior is influenced by EV-transported miRNAs. The treatment of BLM cells with EVs derived from BLM-HI cells resulted in a significantly enhanced invasive capacity, as observed in Matrigel-embedded spheroids and in 2D Boyden chamber assays, with a dose-dependent effect. Conversely, the invasive capacity of BLM cells was reduced when secretion of EVs was inhibited by a sphingomyelinase inhibitor. To investigate the molecular mechanisms behind this effect, we performed next-generation sequencing and identified an enrichment of miR-1246 in these EVs. In functional analyses we demonstrated that both the EV mediated delivery of miR-1246 as well as overexpression contributes to the enhanced invasiveness of BLM cells. We identified a binding site of miR-1246 in the 3’UTR of cyclin G2 (CCNG2) and demonstrated direct binding by a luciferase reporter assay. |
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| DOI: | doi:10.25673/117114 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Resolving-System: https://doi.org/10.25673/117114 |
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| | kostenfrei: Volltext: https://doi.org/10.1186/s12964-024-01820-6 |
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| | kostenfrei: Volltext: https://biosignaling.biomedcentral.com/articles/10.1186/s12964-024-01820-6 |
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| | DOI: https://doi.org/10.25673/117114 |
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| | DOI: https://doi.org/10.1186/s12964-024-01820-6 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Extracellular vesicles |
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| | Invasion |
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| | Melanoma |
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| | miRNAs |
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| K10plus-PPN: | 1906010226 |
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| Verknüpfungen: | → Zeitschrift |
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Extracellular vesicles from highly invasive melanoma subpopulations increase the invasive capacity of less invasive melanoma cells through mir-1246-mediated inhibition of CCNG2 / Kingreen, Tim [VerfasserIn]; 16 September 2024 (Online-Ressource)
