| |  Online-Ressource |
|---|
| Verfasst von: | Zong, Xiangang [VerfasserIn]  |
|---|
| | Schreieck, Jürgen [VerfasserIn] |
|---|
| | Mehrke, Gerhard [VerfasserIn] |
|---|
| | Welling, Andrea [VerfasserIn] |
|---|
| | Schuster, Angela [VerfasserIn] |
|---|
| | Bossert, Eva-Maria [VerfasserIn] |
|---|
| | Flockerzi, Veit [VerfasserIn] |
|---|
| | Hofmann, Franz [VerfasserIn] |
|---|
| Titel: | On the regulation of the expressed L-type calcium channel by cAMP-dependent phosphorylation |
|---|
| Verf.angabe: | Xiangang Zong, Jürgen Schreieck, Gerhard Mehrke, Andera Welling, Angela Schuster, Eva Bosse, Veit Flockerzi, Franz Hofmann |
|---|
| E-Jahr: | 1995 |
|---|
| Jahr: | July 1995 |
|---|
| Umfang: | 8 S. |
|---|
| Fussnoten: | Gesehen am 18.02.2025 |
|---|
| Titel Quelle: | Enthalten in: Pflügers Archiv |
|---|
| Ort Quelle: | Berlin : Springer, 1868 |
|---|
| Jahr Quelle: | 1995 |
|---|
| Band/Heft Quelle: | 430(1995), 3, Seite 340-347 |
|---|
| ISSN Quelle: | 1432-2013 |
|---|
| Abstract: | The Ca2+ channel subunits α1C-a and α1C-b were stably expressed in Chinese hamster ovary (CHO) and human embryonic kidney (HEK) 293 cells. The peak Ba2+ current (IBa) of these cells was not affected significantly by internal dialysis with 0.1 mM cAMP-dependent protein kinase inhibitor peptide (mPKI), 25 μM cAMP-dependent protein kinase catalytic subunit (PKA), or a combination of 25 μM PKA and 1 μM okadaic acid. The activity of the α1C-b channel subunit expressed stably in HEK 293 cells was depressed by 1 μM H 89 and was not increased by superfusion with 5 μM forskolin plus 20 μM isobutylmethylxanthine (IBMX). The α1C-a·β2·α2/δ complex was transiently expressed in HEK 293 cells; it was inhibited by internal dialysis of the cells with 1 μM H 89, but was not affected by internal dialysis with mPKI, PKA or microcystin. Internal dialysis of cells expressing the α1C-a·β2·α2/δ channel with 10 μM PKA did not induce facilitation after a 150-ms prepulse to +50 mV. The Ca2+ current (ICa) of cardiac myocytes increased threefold during internal dialysis with 5 μM PKA or 25 μM microcystin and during external superfusion with 0.1 μM isoproterenol or 5 μM forskolin plus 50 μM IBMX. These results indicate that the L-type Ca2+ channel expressed is not modulated by cAMP-dependent phosphorylation to the same extent as in native cardiac myocytes. |
|---|
| DOI: | doi:10.1007/BF00373908 |
|---|
| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1007/BF00373908 |
|---|
| | DOI: https://doi.org/10.1007/BF00373908 |
|---|
| Datenträger: | Online-Ressource |
|---|
| Sprache: | eng |
|---|
| Sach-SW: | cAMP-dependent regulation of calcium channels |
|---|
| | CHO cells |
|---|
| | HEK 293 cells |
|---|
| | L-Type calcium channels |
|---|
| | Transient and stable expression of calcium channels |
|---|
| K10plus-PPN: | 1917434707 |
|---|
| Verknüpfungen: | → Zeitschrift |
|---|
On the regulation of the expressed L-type calcium channel by cAMP-dependent phosphorylation / Zong, Xiangang [VerfasserIn]; July 1995 (Online-Ressource)
