| |  Online-Ressource |
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| Verfasst von: | Gerke, Carolin [VerfasserIn]  |
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| | Bauersfeld, Liane [VerfasserIn] |
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| | Schirmeister, Ivo [VerfasserIn] |
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| | Mireisz, Chiara Noemi-Marie [VerfasserIn] |
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| | Oberhardt, Valerie [VerfasserIn] |
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| | Mery, Lea [VerfasserIn] |
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| | Wu, Di [VerfasserIn] |
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| | Jürges, Christopher Sebastian [VerfasserIn] |
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| | Spaapen, Robbert M. [VerfasserIn] |
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| | Mussolino, Claudio [VerfasserIn] |
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| | Le, Vu Thuy Khanh [VerfasserIn] |
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| | Trilling, Mirko [VerfasserIn] |
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| | Dölken, Lars [VerfasserIn] |
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| | Paster, Wolfgang [VerfasserIn] |
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| | Erhard, Florian [VerfasserIn] |
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| | Hofmann, Maike [VerfasserIn] |
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| | Schlosser, Andreas [VerfasserIn] |
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| | Hengel, Hartmut [VerfasserIn] |
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| | Momburg, Frank [VerfasserIn] |
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| | Halenius, Anne [VerfasserIn] |
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| Titel: | Multimodal HLA-I genotype regulation by human cytomegalovirus US10 and resulting surface patterning |
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| Verf.angabe: | Carolin Gerke, Liane Bauersfeld, Ivo Schirmeister, Chiara Noemi-Marie Mireisz, Valerie Oberhardt, Lea Mery, Di Wu, Christopher Sebastian Jürges, Robbert M. Spaapen, Claudio Mussolino, Vu Thuy Khanh Le-Trilling, Mirko Trilling, Lars Dölken, Wolfgang Paster, Florian Erhard, Maike Hofmann, Andreas Schlosser, Hartmut Hengel, Frank Momburg, Anne Halenius |
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| E-Jahr: | 2024 |
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| Jahr: | 20 June, 2024 |
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| Umfang: | 29 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | Gesehen am 20.02.2025 |
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| Titel Quelle: | Enthalten in: eLife |
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| Ort Quelle: | Cambridge : eLife Sciences Publications, 2012 |
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| Jahr Quelle: | 2024 |
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| Band/Heft Quelle: | 13(2024) vom: Juni, Artikel-ID e85560, Seite 1-29 |
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| ISSN Quelle: | 2050-084X |
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| Abstract: | Human leucocyte antigen class I (HLA-I) molecules play a central role for both NK and T-cell responses that prevent serious human cytomegalovirus (HCMV) disease. To create opportunities for viral spread, several HCMV-encoded immunoevasins employ diverse strategies to target HLA-I. Among these, the glycoprotein US10 is so far insufficiently studied. While it was reported that US10 interferes with HLA-G expression, its ability to manipulate classical HLA-I antigen presentation remains unknown. In this study, we demonstrate that US10 recognizes and binds to all HLA-I (HLA-A, -B, -C, -E, -G) heavy chains. Additionally, impaired recruitment of HLA-I to the peptide loading complex was observed. Notably, the associated effects varied significantly dependending on HLA-I genotype and allotype: (i) HLA-A molecules evaded downregulation by US10, (ii) tapasin-dependent HLA-B molecules showed impaired maturation and cell surface expression, and (iii) β2m-assembled HLA-C, in particular HLA-C*05:01 and -C*12:03, and HLA-G were strongly retained in complex with US10 in the endoplasmic reticulum. These genotype-specific effects on HLA-I were confirmed through unbiased HLA-I ligandome analyses. Furthermore, in HCMV-infected fibroblasts inhibition of overlapping US10 and US11 transcription had little effect on HLA-A, but induced HLA-B antigen presentation. Thus, the US10-mediated impact on HLA-I results in multiple geno- and allotypic effects in a so far unparalleled and multimodal manner. |
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| DOI: | doi:10.7554/eLife.85560 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.7554/eLife.85560 |
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| | DOI: https://doi.org/10.7554/eLife.85560 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Antigen Presentation |
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| | Cytomegalovirus |
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| | Gene Expression Regulation |
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| | Genotype |
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| | Histocompatibility Antigens Class I |
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| | Host-Pathogen Interactions |
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| | human |
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| | human cytomegalovirus |
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| | Humans |
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| | immunoevasin |
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| | infectious disease |
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| | MHC class I |
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| | microbiology |
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| | peptide loading complex |
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| | Protein Binding |
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| | tapasin |
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| | US10 |
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| | Viral Proteins |
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| | viruses |
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| K10plus-PPN: | 1917688431 |
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| Verknüpfungen: | → Zeitschrift |
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Multimodal HLA-I genotype regulation by human cytomegalovirus US10 and resulting surface patterning / Gerke, Carolin [VerfasserIn]; 20 June, 2024 (Online-Ressource)
