Interleukin-1-induced growth factor expression in postmitotic and resting fibroblasts.

• Verfasst von: Nicole Maas-Szabowski, Nicole [VerfasserIn]
• Verfasst von: Fusenig, Norbert [VerfasserIn]
• Titel: Interleukin-1-induced growth factor expression in postmitotic and resting fibroblasts.
• Verf.angabe: Nicole Maas-Szabowski, Norbert E. Fusenig
• E-Jahr: 1996
• Jahr: December 1996
• Umfang: 7 S.
• Fussnoten: Elektronische Reproduktion der Druck-Ausgabe 4. Januar 2016 ; Gesehen am 24.02.2025
• Titel Quelle: Enthalten in: The journal of investigative dermatology
• Ort Quelle: Amsterdam : Elsevier, 1938
• Jahr Quelle: 1996
• Band/Heft Quelle: 107(1996), 6, Seite 849-855
• ISSN Quelle: 1523-1747
• DOI: doi:10.1111/1523-1747.ep12331158
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: collagen matrix
• Sach-SW: cytokines
• Sach-SW: irradiation
• Sach-SW: reverse transcriptase PCR
• K10plus-PPN: 1917885253

Abstract

Tissue homeostasis in skin is regulated by epithelial-mesenchymal interactions, mostly operating via diffusible factors. To study the underlying regulatory mechanisms, in vitro systems have been established to mimic the in vivo situation in skin. In co-cultures, keratinocytes grow either adjacent to irradiated fibroblasts on plastic or on top of collagen gels containing fibroblasts, thus forming 3-dimensional organotypic structures. Keratinocyte growth is supported in part by fibroblast-produced factors induced by keratinocyte mediators such as interleukin-1 (IL-1). To better understand this cellular interaction and its modulation by fibroblast proliferation and extracellular matrix (ECM), we examined the effect of IL-1 on growth factor expression in proliferating and growth-arrested x-irradiated human dermal fibroblasts on plastic and in resting cells embedded in collagen gels. By semiquantitative reverse transcriptase PCR, we demonstrated that IL-1α and IL-1β stimulated the expression of KGF, HGF, IL-1&alpaha;, IL-1β, IL-1RI, and IL-8 in fibroblasts regardless of their physiologic condition, whereas that of TGF-β remained unaffected. The constitutive mRNA levels were usually lower in irradiated postmitotic and ECM-embedded cells than in proliferating fibroblasts. Cells responded to stimulation with IL-1 under all three culture conditions, although to different degrees depending on the growth factor. As demonstrated for HGF, IL-8, and IL-1β, the IL-1α-induced mRNA expression was followed by production and secretion of protein in irradiated fibroblasts. Thus, our findings show that resting and growth-inhibited fibroblasts, reflecting more closely the situation in dermis, exhibit lower constitutive growth factor expression levels but characteristically respond to IL-1 stimulation.