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Verfasst von:Nicole Maas-Szabowski, Nicole [VerfasserIn]   i
 Fusenig, Norbert [VerfasserIn]   i
Titel:Interleukin-1-induced growth factor expression in postmitotic and resting fibroblasts.
Verf.angabe:Nicole Maas-Szabowski, Norbert E. Fusenig
E-Jahr:1996
Jahr:December 1996
Umfang:7 S.
Fussnoten:Elektronische Reproduktion der Druck-Ausgabe 4. Januar 2016 ; Gesehen am 24.02.2025
Titel Quelle:Enthalten in: The journal of investigative dermatology
Ort Quelle:Amsterdam : Elsevier, 1938
Jahr Quelle:1996
Band/Heft Quelle:107(1996), 6, Seite 849-855
ISSN Quelle:1523-1747
Abstract:Tissue homeostasis in skin is regulated by epithelial-mesenchymal interactions, mostly operating via diffusible factors. To study the underlying regulatory mechanisms, in vitro systems have been established to mimic the in vivo situation in skin. In co-cultures, keratinocytes grow either adjacent to irradiated fibroblasts on plastic or on top of collagen gels containing fibroblasts, thus forming 3-dimensional organotypic structures. Keratinocyte growth is supported in part by fibroblast-produced factors induced by keratinocyte mediators such as interleukin-1 (IL-1). To better understand this cellular interaction and its modulation by fibroblast proliferation and extracellular matrix (ECM), we examined the effect of IL-1 on growth factor expression in proliferating and growth-arrested x-irradiated human dermal fibroblasts on plastic and in resting cells embedded in collagen gels. By semiquantitative reverse transcriptase PCR, we demonstrated that IL-1α and IL-1β stimulated the expression of KGF, HGF, IL-1&alpaha;, IL-1β, IL-1RI, and IL-8 in fibroblasts regardless of their physiologic condition, whereas that of TGF-β remained unaffected. The constitutive mRNA levels were usually lower in irradiated postmitotic and ECM-embedded cells than in proliferating fibroblasts. Cells responded to stimulation with IL-1 under all three culture conditions, although to different degrees depending on the growth factor. As demonstrated for HGF, IL-8, and IL-1β, the IL-1α-induced mRNA expression was followed by production and secretion of protein in irradiated fibroblasts. Thus, our findings show that resting and growth-inhibited fibroblasts, reflecting more closely the situation in dermis, exhibit lower constitutive growth factor expression levels but characteristically respond to IL-1 stimulation.
DOI:doi:10.1111/1523-1747.ep12331158
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1111/1523-1747.ep12331158
 Volltext: https://www.sciencedirect.com/science/article/pii/S0022202X1542754X
 DOI: https://doi.org/10.1111/1523-1747.ep12331158
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:collagen matrix
 cytokines
 irradiation
 reverse transcriptase PCR
K10plus-PPN:1917885253
Verknüpfungen:→ Zeitschrift

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