| Online-Ressource |
Verfasst von: | Nicole Maas-Szabowski, Nicole [VerfasserIn]  |
| Fusenig, Norbert [VerfasserIn]  |
Titel: | Interleukin-1-induced growth factor expression in postmitotic and resting fibroblasts. |
Verf.angabe: | Nicole Maas-Szabowski, Norbert E. Fusenig |
E-Jahr: | 1996 |
Jahr: | December 1996 |
Umfang: | 7 S. |
Fussnoten: | Elektronische Reproduktion der Druck-Ausgabe 4. Januar 2016 ; Gesehen am 24.02.2025 |
Titel Quelle: | Enthalten in: The journal of investigative dermatology |
Ort Quelle: | Amsterdam : Elsevier, 1938 |
Jahr Quelle: | 1996 |
Band/Heft Quelle: | 107(1996), 6, Seite 849-855 |
ISSN Quelle: | 1523-1747 |
Abstract: | Tissue homeostasis in skin is regulated by epithelial-mesenchymal interactions, mostly operating via diffusible factors. To study the underlying regulatory mechanisms, in vitro systems have been established to mimic the in vivo situation in skin. In co-cultures, keratinocytes grow either adjacent to irradiated fibroblasts on plastic or on top of collagen gels containing fibroblasts, thus forming 3-dimensional organotypic structures. Keratinocyte growth is supported in part by fibroblast-produced factors induced by keratinocyte mediators such as interleukin-1 (IL-1). To better understand this cellular interaction and its modulation by fibroblast proliferation and extracellular matrix (ECM), we examined the effect of IL-1 on growth factor expression in proliferating and growth-arrested x-irradiated human dermal fibroblasts on plastic and in resting cells embedded in collagen gels. By semiquantitative reverse transcriptase PCR, we demonstrated that IL-1α and IL-1β stimulated the expression of KGF, HGF, IL-1&alpaha;, IL-1β, IL-1RI, and IL-8 in fibroblasts regardless of their physiologic condition, whereas that of TGF-β remained unaffected. The constitutive mRNA levels were usually lower in irradiated postmitotic and ECM-embedded cells than in proliferating fibroblasts. Cells responded to stimulation with IL-1 under all three culture conditions, although to different degrees depending on the growth factor. As demonstrated for HGF, IL-8, and IL-1β, the IL-1α-induced mRNA expression was followed by production and secretion of protein in irradiated fibroblasts. Thus, our findings show that resting and growth-inhibited fibroblasts, reflecting more closely the situation in dermis, exhibit lower constitutive growth factor expression levels but characteristically respond to IL-1 stimulation. |
DOI: | doi:10.1111/1523-1747.ep12331158 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1111/1523-1747.ep12331158 |
| Volltext: https://www.sciencedirect.com/science/article/pii/S0022202X1542754X |
| DOI: https://doi.org/10.1111/1523-1747.ep12331158 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | collagen matrix |
| cytokines |
| irradiation |
| reverse transcriptase PCR |
K10plus-PPN: | 1917885253 |
Verknüpfungen: | → Zeitschrift |
Interleukin-1-induced growth factor expression in postmitotic and resting fibroblasts. / Nicole Maas-Szabowski, Nicole [VerfasserIn]; December 1996 (Online-Ressource)