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| Verfasst von: | Ylade, Michelle [VerfasserIn]  |
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| | Aziz, Asma Binte [VerfasserIn] |
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| | Daag, Jedas Veronica [VerfasserIn] |
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| | Crisostomo, Maria Vinna [VerfasserIn] |
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| | Agrupis, Kristal-An [VerfasserIn] |
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| | Maronilla, Maria Angela [VerfasserIn] |
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| | Hong, Chloe Sye Lim [VerfasserIn] |
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| | Kim, Hwa Young [VerfasserIn] |
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| | Njau, Irene [VerfasserIn] |
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| | Lopez, March Helena Jane [VerfasserIn] |
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| | Deen, Jacqueline [VerfasserIn] |
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| | Kim, Deok Ryun [VerfasserIn] |
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| | You, Young Ae [VerfasserIn] |
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| | Kang, Sophie S. Y. [VerfasserIn] |
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| | Marks, Florian [VerfasserIn] |
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| | Tadesse, Birkneh Tilahun [VerfasserIn] |
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| Titel: | Using dried blood spots to quantitatively detect anti-SARS-CoV-2 IgG antibodies by ELISA |
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| Titelzusatz: | a validation study |
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| Verf.angabe: | Michelle Ylade, Asma Binte Aziz, Jedas Veronica Daag, Maria Vinna Crisostomo, Kristal-An Agrupis, Maria Angela Maronilla, Chloe Sye Lim Hong, Hwa Young Kim, Irene Njau, March Helena Jane Lopez, Jacqueline Deen, Deok Ryun Kim, Young Ae You, Sophie S.Y. Kang, Florian Marks, and Birkneh Tilahun Tadesse |
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| E-Jahr: | 2024 |
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| Jahr: | 25 Jun 2024 |
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| Umfang: | 5 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | Gesehen am 03.03.2025 |
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| Titel Quelle: | Enthalten in: The American journal of tropical medicine and hygiene |
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| Ort Quelle: | Northbrook, Ill. : American Soc. of Tropical Medicine and Hygiene, 1921 |
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| Jahr Quelle: | 2024 |
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| Band/Heft Quelle: | 111(2024), 2, Seite 391-395 |
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| ISSN Quelle: | 1476-1645 |
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| Abstract: | ABSTRACT. SARS-CoV-2 serological testing is useful to determine seroprevalence, epidemiological trends, and the extent of transmission. The collection and transport of serum samples can be logistically challenging, especially in remote underserved areas. Dried blood spots (DBSs) would allow easier sample collection and logistical handling compared with standard serum collection, particularly for extensive and repeated SARS-CoV-2 serosurveys. We evaluated the sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of the IgG ELISA (Wantai, Beijing, China) using DBSs against sera for the quantitative detection of SARS-CoV-2 IgG antibody. The IgG ELISA was used to test paired sera and DBSs obtained from individuals with recent virologically confirmed COVID-19 illness and banked paired sera and DBSs collected before the COVID-19 pandemic. We found that 100/100 (100%) seropositive samples were positive using DBSs, and 193/194 (99%) seronegative samples were negative using DBSs. Compared with sera, the DBS method had a 100% sensitivity, 99% specificity, 99% PPV, and 100% NPV. Use of DBSs for SARS-CoV-2 household or population serosurveys may be considered in situations with limitations in sample collection, shipment, and storage. |
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| DOI: | doi:10.4269/ajtmh.23-0306 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.4269/ajtmh.23-0306 |
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| | kostenfrei: Volltext: https://www.ajtmh.org/view/journals/tpmd/111/2/article-p391.xml |
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| | DOI: https://doi.org/10.4269/ajtmh.23-0306 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1918875715 |
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| Verknüpfungen: | → Zeitschrift |
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Using dried blood spots to quantitatively detect anti-SARS-CoV-2 IgG antibodies by ELISA / Ylade, Michelle [VerfasserIn]; 25 Jun 2024 (Online-Ressource)
