| |  Online-Ressource |
|---|
| Verfasst von: | Buglakova, Elena [VerfasserIn]  |
|---|
| | Ekelöf, Måns [VerfasserIn] |
|---|
| | Schwaiger-Haber, Michaela [VerfasserIn] |
|---|
| | Schlicker, Lisa [VerfasserIn] |
|---|
| | Molenaar, Martijn R. [VerfasserIn] |
|---|
| | Shahraz, Mohammed [VerfasserIn] |
|---|
| | Stuart, Lachlan [VerfasserIn] |
|---|
| | Eisenbarth, Andreas [VerfasserIn] |
|---|
| | Hilsenstein, Volker [VerfasserIn] |
|---|
| | Patti, Gary J. [VerfasserIn] |
|---|
| | Schulze, Almut [VerfasserIn] |
|---|
| | Snæbjörnsson, Marteinn [VerfasserIn] |
|---|
| | Alexandrov, Theodore [VerfasserIn] |
|---|
| Titel: | Spatial single-cell isotope tracing reveals heterogeneity of de novo fatty acid synthesis in cancer |
|---|
| Verf.angabe: | Elena Buglakova, Måns Ekelöf, Michaela Schwaiger-Haber, Lisa Schlicker, Martijn R. Molenaar, Mohammed Shahraz, Lachlan Stuart, Andreas Eisenbarth, Volker Hilsenstein, Gary J. Patti, Almut Schulze, Marteinn T. Snaebjornsson & Theodore Alexandrov |
|---|
| E-Jahr: | 2024 |
|---|
| Jahr: | 09 September 2024 |
|---|
| Umfang: | 25 S. |
|---|
| Illustrationen: | Illustrationen |
|---|
| Fussnoten: | Gesehen am 07.03.2025 |
|---|
| Titel Quelle: | Enthalten in: Nature metabolism |
|---|
| Ort Quelle: | [London] : Springer Nature, 2019 |
|---|
| Jahr Quelle: | 2024 |
|---|
| Band/Heft Quelle: | 6(2024), 9, Seite 1695-1711 |
|---|
| ISSN Quelle: | 2522-5812 |
|---|
| Abstract: | While heterogeneity is a key feature of cancer, understanding metabolic heterogeneity at the single-cell level remains a challenge. Here we present 13C-SpaceM, a method for spatial single-cell isotope tracing that extends the previously published SpaceM method with detection of 13C6-glucose-derived carbons in esterified fatty acids. We validated 13C-SpaceM on spatially heterogeneous models using liver cancer cells subjected to either normoxia-hypoxia or ATP citrate lyase depletion. This revealed substantial single-cell heterogeneity in labelling of the lipogenic acetyl-CoA pool and in relative fatty acid uptake versus synthesis hidden in bulk analyses. Analysing tumour-bearing brain tissue from mice fed a 13C6-glucose-containing diet, we found higher glucose-dependent synthesis of saturated fatty acids and increased elongation of essential fatty acids in tumours compared with healthy brains. Furthermore, our analysis uncovered spatial heterogeneity in lipogenic acetyl-CoA pool labelling in tumours. Our method enhances spatial probing of metabolic activities in single cells and tissues, providing insights into fatty acid metabolism in homoeostasis and disease. |
|---|
| DOI: | doi:10.1038/s42255-024-01118-4 |
|---|
| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1038/s42255-024-01118-4 |
|---|
| | kostenfrei: Volltext: https://www.nature.com/articles/s42255-024-01118-4 |
|---|
| | DOI: https://doi.org/10.1038/s42255-024-01118-4 |
|---|
| Datenträger: | Online-Ressource |
|---|
| Sprache: | eng |
|---|
| Sach-SW: | Biological techniques |
|---|
| | Metabolomics |
|---|
| K10plus-PPN: | 1919290249 |
|---|
| Verknüpfungen: | → Zeitschrift |
|---|
Spatial single-cell isotope tracing reveals heterogeneity of de novo fatty acid synthesis in cancer / Buglakova, Elena [VerfasserIn]; 09 September 2024 (Online-Ressource)
