Measurement of blood flow in the main arteriole of the villi in rat small iIntestine with FITC-labeled erythrocytes

• Verfasst von: Ruh, Joachim [VerfasserIn]
• Verfasst von: Ryschich, Eduard [VerfasserIn]
• Verfasst von: Secchi, Andreas [VerfasserIn]
• Verfasst von: Gebhard, Martha-Maria [VerfasserIn]
• Verfasst von: Glaser, Florian [VerfasserIn]
• Verfasst von: Klar, Ernst [VerfasserIn]
• Verfasst von: Herfarth, Christian [VerfasserIn]
• Titel: Measurement of blood flow in the main arteriole of the villi in rat small iIntestine with FITC-labeled erythrocytes
• Verf.angabe: Joachim Ruh, Eduard Ryschich, Andreas Secchi, Martha Maria Gebhard, Florian Glaser, Ernst Klar, and Christian Herfarth
• E-Jahr: 1998
• Jahr: July 1998
• Umfang: 8 S.
• Fussnoten: Elektronische Reproduktion der Druck-Ausgabe 25. Mai 2002 ; Gesehen am 12.03.2025
• Titel Quelle: Enthalten in: Microvascular research
• Ort Quelle: Orlando, Fla. : Academic Press, 1968
• Jahr Quelle: 1998
• Band/Heft Quelle: 56(1998), 1, Seite 62-69
• ISSN Quelle: 1095-9319
• DOI: doi:10.1006/mvre.1998.2082
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: main arteriole
• Sach-SW: microcirculation
• Sach-SW: rat small intestine
• Sach-SW: villus
• K10plus-PPN: 1919606084

Abstract

Changes of blood flow in the intestine occur under various pathological conditions. The mucosa of the intestine is especially sensitive to tissue damage resulting in swelling, loss of tissue integrity, and ulceration. Changes of blood supply to the mucosa may contribute to local tissue damage. Therefore, the quantification of the perfusion of the intestinal mucosa in an animal model may help to elucidate the involved pathophysiological mechanisms. In our study, autologous erythrocytes were labeled with fluorescein-isothiocyanate and used for the evaluation of erythrocyte velocity in the main arteriole of the villi in the distal part of the ileum using intravital microscopy. In addition, the arteriolar diameter was determined, and the arteriolar blood flow was calculated. Under stable cardiovascular and respiratory conditions, blood flow ranged between 6.6 ± 0.3 and 5.9 ± 0.3 nl/min (means ± SEM) during the observation period of 120 min. Our results suggest that this approach is a feasible method to quantify blood flow in the main arteriole of the villi and is therefore a suitable method for further investigating changes of mucosal blood flow in acute and chronic states of bowel disease.