Glyco-STORM: nanoscale mapping of the cellular glycosylation landscape

• Verfasst von: Schröter, Helene Gregoria [VerfasserIn]
• Verfasst von: Saß, Steffen [VerfasserIn]
• Verfasst von: Heilemann, Mike [VerfasserIn]
• Verfasst von: Kuner, Thomas [VerfasserIn]
• Verfasst von: Klevanski, Maja [VerfasserIn]
• Titel: Glyco-STORM: nanoscale mapping of the cellular glycosylation landscape
• Verf.angabe: Helene Gregoria Schroeter, Steffen Sass, Mike Heilemann, Thomas Kuner, Maja Klevanski
• E-Jahr: 2025
• Jahr: February 1, 2025
• Umfang: 38 S.
• Illustrationen: Illustrationen
• Fussnoten: Gesehen am 17.03.2025
• Titel Quelle: Enthalten in: bioRxiv beta
• Ort Quelle: Cold Spring Harbor : Cold Spring Harbor Laboratory, NY, 2013
• Jahr Quelle: 2025
• Band/Heft Quelle: (2025) vom: Feb., Artikel-ID 2025.02.19.639131, Seite 1-38
• DOI: doi:10.1101/2025.02.19.639131
• Datenträger: Online-Ressource
• Sprache: eng
• Bibliogr. Hinweis: Forschungsdaten: Schröter, Helene Gregoria: Glyco-STORM: nanoscale mapping of the cellular glycosylation landscape
• K10plus-PPN: 1919875654

Abstract

Glycosylation is a crucial biochemical modification of proteins and other biomolecules in cells that generates an exceptional structural and functional diversity. Aberrant glycosylation is implicated in numerous diseases, including neurodegenerative disorders and cancer. Despite its significance, methodological constraints to date have limited the exploration of the nanometer scale spatial arrangement of glycans across entire cells. We developed Glyco-STORM, a super-resolution imaging approach that generates nano-structural maps of cellular glycosylation. Glyco-STORM employs fluorophore-labeled lectins and multiplexed single-molecule super-resolution microscopy, in combination with nanoscale spatial pattern analysis. For example, Glyco-STORM unraveled nanodomains within the endoplasmic reticulum, subdomains along the Golgi axes, and a polarized lysosomal clathrin coat. At synaptic contact sites, mature glycans delineate the synaptic cleft and subsynaptic tubules adjacent to the postsynaptic density. In summary, Glyco-STORM elucidates the spatial arrangement of glycosylation sites from subcellular to molecular levels, revealing the previously obscured glycosylation landscape at nanoscale and establishing a ’spatial glycosylation code’ that provides a unique perspective on cellular organization distinct from traditional protein-centric views.