| |  Online-Ressource |
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| Verfasst von: | Petrich, Annett [VerfasserIn]  |
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| | Hwang, Gyu Min [VerfasserIn] |
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| | La Rocca, Laetitia [VerfasserIn] |
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| | Hassan, Mariam [VerfasserIn] |
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| | Anders-Ößwein, Maria [VerfasserIn] |
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| | Sonntag-Buck, Vera [VerfasserIn] |
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| | Heuser, Anke-Mareil [VerfasserIn] |
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| | Laketa, Vibor [VerfasserIn] |
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| | Müller, Barbara [VerfasserIn] |
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| | Kräusslich, Hans-Georg [VerfasserIn] |
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| | Klaus, Severina [VerfasserIn] |
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| Titel: | Expanding insights |
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| Titelzusatz: | harnessing expansion microscopy for super-resolution analysis of HIV-1-cell interactions |
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| Verf.angabe: | Annett Petrich, Gyu Min Hwang, Laetitia La Rocca, Mariam Hassan, Maria Anders-Össwein, Vera Sonntag-Buck, Anke-Mareil Heuser, Vibor Laketa, Barbara Müller, Hans-Georg Kräusslich and Severina Klaus |
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| E-Jahr: | 2024 |
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| Jahr: | 15 October 2024 |
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| Umfang: | 26 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | Gesehen am 24.03.2025 |
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| Titel Quelle: | Enthalten in: Viruses |
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| Ort Quelle: | Basel : MDPI, 2009 |
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| Jahr Quelle: | 2024 |
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| Band/Heft Quelle: | 16(2024), 10, Artikel-ID 1610, Seite 1-26 |
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| ISSN Quelle: | 1999-4915 |
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| Abstract: | Expansion microscopy has recently emerged as an alternative technique for achieving high-resolution imaging of biological structures. Improvements in resolution are achieved by physically expanding samples through embedding in a swellable hydrogel before microscopy. However, expansion microscopy has been rarely used in the field of virology. Here, we evaluate and characterize the ultrastructure expansion microscopy (U-ExM) protocol, which facilitates approximately four-fold sample expansion, enabling the visualization of different post-entry stages of the HIV-1 life cycle, focusing on nuclear events. Our findings demonstrate that U-ExM provides robust sample expansion and preservation across different cell types, including cell-culture-adapted and primary CD4+ T-cells as well as monocyte-derived macrophages, which are known HIV-1 reservoirs. Notably, cellular targets such as nuclear bodies and the chromatin landscape remain well preserved after expansion, allowing for detailed investigation of HIV-1-cell interactions at high resolution. Our data indicate that morphologically distinct HIV-1 capsid assemblies can be differentiated within the nuclei of infected cells and that U-ExM enables detection of targets that are masked in commonly used immunofluorescence protocols. In conclusion, we advocate for U-ExM as a valuable new tool for studying virus-host interactions with enhanced spatial resolution. |
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| DOI: | doi:10.3390/v16101610 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.3390/v16101610 |
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| | kostenfrei: Volltext: https://www.mdpi.com/1999-4915/16/10/1610 |
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| | DOI: https://doi.org/10.3390/v16101610 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | expansion microscopy |
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| | HIV-1 |
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| | HIV-1 capsid |
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| | HIV-1 nuclear import |
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| | HIV-1 post-entry |
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| | super-resolution microscopy |
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| | ultrastructure expansion microscopy |
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| | virus-host interaction |
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| K10plus-PPN: | 1920388125 |
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| Verknüpfungen: | → Zeitschrift |
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Expanding insights / Petrich, Annett [VerfasserIn]; 15 October 2024 (Online-Ressource)
