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| Verfasst von: | Ishikawa-Ankerhold, Hellen [VerfasserIn]  |
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| | Busch, Benjamin [VerfasserIn] |
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| | Bader, Almke [VerfasserIn] |
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| | Maier-Begandt, Daniela [VerfasserIn] |
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| | Dionisio, Flavio [VerfasserIn] |
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| | Namineni, Sukumar [VerfasserIn] |
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| | Vladymyrov, Mykhailo [VerfasserIn] |
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| | Harrison, Ute [VerfasserIn] |
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| | Heuvel, Dominic van den [VerfasserIn] |
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| | Tomas, Lukas [VerfasserIn] |
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| | Walzog, Barbara [VerfasserIn] |
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| | Massberg, Steffen [VerfasserIn] |
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| | Schulz, Christian [VerfasserIn] |
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| | Haas, Rainer [VerfasserIn] |
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| Titel: | Novel multiphoton intravital imaging enables real-time study of Helicobacter pylori interaction with neutrophils and macrophages in the mouse stomach |
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| Verf.angabe: | Hellen Ishikawa-Ankerhold, Benjamin Busch, Almke Bader, Daniela Maier-Begandt, Flavio Dionisio, Sukumar Namineni, Mykhailo Vladymyrov, Ute Harrison, Dominic van den Heuvel, Lukas Tomas, Barbara Walzog, Steffen Massberg, Christian Schulz, Rainer Haas |
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| E-Jahr: | 2024 |
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| Jahr: | September 30, 2024 |
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| Umfang: | 21 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | Gesehen am 03.04.2025 |
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| Titel Quelle: | Enthalten in: Public Library of SciencePLoS pathogens |
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| Ort Quelle: | Lawrence, Kan. : PLoS, 2005 |
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| Jahr Quelle: | 2024 |
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| Band/Heft Quelle: | 20(2024), 9, Artikel-ID e1012580, Seite 1-21 |
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| ISSN Quelle: | 1553-7374 |
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| Abstract: | Helicobacter pylori (H. pylori) is a bacterial pathogen that exclusively colonizes the human gastric mucosa and can cause persistent infection. In this process, H. pylori employs various strategies to avoid recognition by the human immune system. These range from passive defense strategies (e.g., altered LPS or flagellin structures) that prevent recognition by pattern recognition receptors (PRRs) to more active approaches, such as inhibition of IL-2 secretion and proliferation of T cells via VacA. Despite the growing evidence that H. pylori actively manipulates the human immune system for its own benefit, the direct interaction of H. pylori with immune cells in situ is poorly studied. Here, we present a novel intravital imaging model of the murine stomach gastric mucosa and show for the first time the in situ recruitment of neutrophils during infection and a direct H. pylori-macrophage interaction. For this purpose, we applied multiphoton intravital microscopy adapted with live drift correction software (VivoFollow) on LysM-eGFP and CX3CR1-eGFP reporter mice strains in which specific subsets of leukocytes are fluorescently labeled. Multiphoton microscopy is proving to be an excellent tool for characterizing interactions between immune cells and pathogens in vivo. |
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| DOI: | doi:10.1371/journal.ppat.1012580 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1371/journal.ppat.1012580 |
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| | kostenfrei: Volltext: https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1012580 |
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| | DOI: https://doi.org/10.1371/journal.ppat.1012580 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Computer software |
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| | Gastric mucosa |
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| | Gastrointestinal infections |
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| | Immune cells |
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| | In vivo imaging |
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| | Macrophages |
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| | Neutrophils |
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| | Stomach |
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| K10plus-PPN: | 1921317566 |
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| Verknüpfungen: | → Zeitschrift |
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Novel multiphoton intravital imaging enables real-time study of Helicobacter pylori interaction with neutrophils and macrophages in the mouse stomach / Ishikawa-Ankerhold, Hellen [VerfasserIn]; September 30, 2024 (Online-Ressource)
