Sequence analysis of hepatitis GB virus C (GBV-C) isolates from 14 patients

• Verfasst von: Seipp, Stefanie [VerfasserIn]
• Verfasst von: Wahl, Rafael [VerfasserIn]
• Verfasst von: Müller, Hubert [VerfasserIn]
• Verfasst von: Stremmel, Wolfgang [VerfasserIn]
• Verfasst von: Theilmann, Lorenz [VerfasserIn]
• Verfasst von: Goeser, Tobias [VerfasserIn]
• Titel: Sequence analysis of hepatitis GB virus C (GBV-C) isolates from 14 patients
• Verf.angabe: Stefanie Seipp, Rafael Wahl, Hubert Mueller, Wolfgang Stremmel, Lorenz Theilmann, Tobias Goeser
• E-Jahr: 1996
• Jahr: 2 December 1996
• Umfang: 8 S.
• Fussnoten: Elektronische Reproduktion der Druck-Ausgabe 6. Oktober 1998 ; Gesehen am 17.04.2025
• Titel Quelle: Enthalten in: Virus research
• Ort Quelle: Amsterdam [u.a.] : Elsevier Science, 1984
• Jahr Quelle: 1996
• Band/Heft Quelle: 46(1996), 1, Seite 81-88
• ISSN Quelle: 1872-7492
• DOI: doi:10.1016/S0168-1702(96)01377-9
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: GBV-C
• Sach-SW: Genome diversity
• Sach-SW: Hepatitis GB-C virus
• Sach-SW: Sequence analysis
• K10plus-PPN: 192320632X

Abstract

In 1995, a new human hepatitis virus belonging to the family Flaviviridae was described and designated hepatitis GBV-C. To investigate variations within the genome of GBV-C and to study the relationship of GBV-C to GBV-A/B or hepatitis C virus (HCV), we established a detection system using reverse transcriptase polymerase chain reaction (RT-PCR) of the putative helicase region (NS3). So far, isolates derived from 14 different GBV-C-positive sera were analyzed (GBV-C/S3-36), showing 80.1-89.4% (mean: 85%) identical nucleotides. The deduced amino acid sequences revealed 97.3% homology. Nucleotide sequences of GBV-C/S3-36 revealed about 60% identity to GBV-A as well as to HCV, but only 56% identity to GBV-B. Amino acid sequences revealed 73.4 and 68.6% similarity to GBV-A and GBV-B, respectively, but a slightly higher percentage of 78.5% to HCV sequences. Thus, according to the putative GBV-C helicase sequence, a subtyping of GBV-C into different genotypes may be necessary.