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| Verfasst von: | Valeriano Nunes, Ivanéia [VerfasserIn]  |
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| | Breitenbach, Luisa [VerfasserIn] |
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| | Pawusch, Sarah [VerfasserIn] |
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| | Eigenbrod, Tatjana [VerfasserIn] |
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| | Ananth, Swetha [VerfasserIn] |
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| | Schad, Paulina [VerfasserIn] |
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| | Fackler, Oliver Till [VerfasserIn] |
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| | Butter, Falk [VerfasserIn] |
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| | Dalpke, Alexander [VerfasserIn] |
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| | Chen, Lan-Sun [VerfasserIn] |
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| Titel: | Bacterial RNA sensing by TLR8 requires RNase 6 processing and is inhibited by RNA 2’O-methylation |
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| Verf.angabe: | Ivanéia V Nunes, Luisa Breitenbach, Sarah Pawusch, Tatjana Eigenbrod, Swetha Ananth, Paulina Schad, Oliver T Fackler, Falk Butter, Alexander H Dalpke & Lan-Sun Chen |
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| E-Jahr: | 2024 |
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| Jahr: | November 2024 |
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| Umfang: | 19 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | Veröffentlicht: 3. Oktober 2024 ; Gesehen am 22.04.2025 |
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| Titel Quelle: | Enthalten in: European Molecular Biology OrganizationEMBO reports |
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| Ort Quelle: | [London] : Nature Publishing Group UK, 2000 |
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| Jahr Quelle: | 2024 |
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| Band/Heft Quelle: | 25(2024), 11 vom: Nov., Seite 4674-4692 |
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| ISSN Quelle: | 1469-3178 |
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| Abstract: | TLR8 senses single-stranded RNA (ssRNA) fragments, processed via cleavage by ribonuclease (RNase) T2 and RNase A family members. Processing by these RNases releases uridines and purine-terminated residues resulting in TLR8 activation. Monocytes show high expression of RNase 6, yet this RNase has not been analyzed for its physiological contribution to the recognition of bacterial RNA by TLR8. Here, we show a role for RNase 6 in TLR8 activation. BLaER1 cells, transdifferentiated into monocyte-like cells, as well as primary monocytes deficient for RNASE6 show a dampened TLR8-dependent response upon stimulation with isolated bacterial RNA (bRNA) and also upon infection with live bacteria. Pretreatment of bacterial RNA with recombinant RNase 6 generates fragments that induce TLR8 stimulation in RNase 6 knockout cells. 2’O-RNA methyl modification, when introduced at the first uridine in the UA dinucleotide, impairs processing by RNase 6 and dampens TLR8 stimulation. In summary, our data show that RNase 6 processes bacterial RNA and generates uridine-terminated breakdown products that activate TLR8. |
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| DOI: | doi:10.1038/s44319-024-00281-9 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1038/s44319-024-00281-9 |
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| | kostenfrei: Volltext: https://www.embopress.org/doi/full/10.1038/s44319-024-00281-9 |
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| | DOI: https://doi.org/10.1038/s44319-024-00281-9 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Innate Immunity |
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| | Nucleic Acid Recognition |
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| | RNA Modifications |
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| | RNase 6 |
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| | Toll-Like Receptor 8 |
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| K10plus-PPN: | 1923419773 |
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| Verknüpfungen: | → Zeitschrift |
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Bacterial RNA sensing by TLR8 requires RNase 6 processing and is inhibited by RNA 2’O-methylation / Valeriano Nunes, Ivanéia [VerfasserIn]; November 2024 (Online-Ressource)
