Disinfection of cell-associated and extracellular HIV-1 by PUVA treatment

• Verfasst von: Deichmann, Martin [VerfasserIn]
• Verfasst von: Sczakiel, Georg [VerfasserIn]
• Verfasst von: Haas, Rainer [VerfasserIn]
• Titel: Disinfection of cell-associated and extracellular HIV-1 by PUVA treatment
• Verf.angabe: Martin Deichmann, Georg Sczakiel, Rainer Haas
• E-Jahr: 1997
• Jahr: October 1997
• Umfang: 7 S.
• Fussnoten: Elektronische Reproduktion der Druck-Ausgabe 5. Februar 1998 ; Gesehen am 07.05.2025
• Titel Quelle: Enthalten in: Journal of virological methods
• Ort Quelle: Amsterdam [u.a.] : Elsevier Science, 1980
• Jahr Quelle: 1997
• Band/Heft Quelle: 68(1997), 1, Seite 89-95
• ISSN Quelle: 1879-0984
• DOI: doi:10.1016/S0166-0934(97)00112-2
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: 4′-aminomethyl-4,5′,8-trimethylpsoralen (AMT)
• Sach-SW: Flow cytometry
• Sach-SW: Human immunodeficiency virus type 1 (HIV-1)
• Sach-SW: Inactivation
• Sach-SW: UVA-irradiation
• K10plus-PPN: 1924932733

Abstract

To inactivate cell-associated and extracellular HIV-1 while preserving cellular surface antigens, a procedure was used based on PUVA treatment i.e. addition of psoralen to cell suspensions followed by irradiation with UVA light. T-lymphoid MT-4 cells were infected with HIV-1 strain NL4-3, 4′-aminomethyl-4,5′,8-trimethylpsoralen was added, and the cell suspension was irradiated with 20 mW/cm2 UVA light for 3, 4 and 5 min. To evaluate virus inactivation, cells and supernatants were diluted serially and cocultured with uninfected MT-4 cells. Infectious HIV-1 was detected by cytopathic effects, immunofluorescence and p24 antigen ELISA. UVA irradiation at 3.6 J/cm2 (3 min 20 mW/cm2) reduced the amounts of both cell-associated and extracellular infectious HIV-1 by more than five orders of magnitude. Even at more stringent conditions of PUVA treatment (10 min 20 mW/cm2 UVA irradiation), conformational cellular surface epitopes remained detectable by flow cytometry.