Status: Bibliographieeintrag
Standort: ---
Exemplare:
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| Online-Ressource |
Verfasst von: | Deichmann, Martin [VerfasserIn]  |
| Sczakiel, Georg [VerfasserIn]  |
| Haas, Rainer [VerfasserIn]  |
Titel: | Disinfection of cell-associated and extracellular HIV-1 by PUVA treatment |
Verf.angabe: | Martin Deichmann, Georg Sczakiel, Rainer Haas |
E-Jahr: | 1997 |
Jahr: | October 1997 |
Umfang: | 7 S. |
Fussnoten: | Elektronische Reproduktion der Druck-Ausgabe 5. Februar 1998 ; Gesehen am 07.05.2025 |
Titel Quelle: | Enthalten in: Journal of virological methods |
Ort Quelle: | Amsterdam [u.a.] : Elsevier Science, 1980 |
Jahr Quelle: | 1997 |
Band/Heft Quelle: | 68(1997), 1, Seite 89-95 |
ISSN Quelle: | 1879-0984 |
Abstract: | To inactivate cell-associated and extracellular HIV-1 while preserving cellular surface antigens, a procedure was used based on PUVA treatment i.e. addition of psoralen to cell suspensions followed by irradiation with UVA light. T-lymphoid MT-4 cells were infected with HIV-1 strain NL4-3, 4′-aminomethyl-4,5′,8-trimethylpsoralen was added, and the cell suspension was irradiated with 20 mW/cm2 UVA light for 3, 4 and 5 min. To evaluate virus inactivation, cells and supernatants were diluted serially and cocultured with uninfected MT-4 cells. Infectious HIV-1 was detected by cytopathic effects, immunofluorescence and p24 antigen ELISA. UVA irradiation at 3.6 J/cm2 (3 min 20 mW/cm2) reduced the amounts of both cell-associated and extracellular infectious HIV-1 by more than five orders of magnitude. Even at more stringent conditions of PUVA treatment (10 min 20 mW/cm2 UVA irradiation), conformational cellular surface epitopes remained detectable by flow cytometry. |
DOI: | doi:10.1016/S0166-0934(97)00112-2 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/S0166-0934(97)00112-2 |
| Volltext: https://www.sciencedirect.com/science/article/pii/S0166093497001122 |
| DOI: https://doi.org/10.1016/S0166-0934(97)00112-2 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | 4′-aminomethyl-4,5′,8-trimethylpsoralen (AMT) |
| Flow cytometry |
| Human immunodeficiency virus type 1 (HIV-1) |
| Inactivation |
| UVA-irradiation |
K10plus-PPN: | 1924932733 |
Verknüpfungen: | → Zeitschrift |
Disinfection of cell-associated and extracellular HIV-1 by PUVA treatment / Deichmann, Martin [VerfasserIn]; October 1997 (Online-Ressource)
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