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| Verfasst von: | Wu, Yuqin [VerfasserIn]  |
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| | Foollee, Ashish [VerfasserIn] |
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| | Chan, Andrea Y. [VerfasserIn] |
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| | Hille, Susanne [VerfasserIn] |
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| | Hauke, Jana [VerfasserIn] |
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| | Challis, Matthew P. [VerfasserIn] |
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| | Johnson, Jared L. [VerfasserIn] |
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| | Yaron, Tomer M. [VerfasserIn] |
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| | Mynard, Victoria [VerfasserIn] |
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| | Aung, Okka H. [VerfasserIn] |
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| | Cleofe, Maria Almira S. [VerfasserIn] |
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| | Huang, Cheng [VerfasserIn] |
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| | Lim Kam Sian, Terry C. C. [VerfasserIn] |
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| | Rahbari, Mohammad [VerfasserIn] |
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| | Gallage, Suchira [VerfasserIn] |
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| | Heikenwälder, Mathias [VerfasserIn] |
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| | Cantley, Lewis C. [VerfasserIn] |
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| | Schittenhelm, Ralf B. [VerfasserIn] |
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| | Formosa, Luke E. [VerfasserIn] |
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| | Smith, Greg C. [VerfasserIn] |
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| | Okun, Jürgen G. [VerfasserIn] |
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| | Müller, Oliver J. [VerfasserIn] |
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| | Rusu, Patricia M. [VerfasserIn] |
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| | Rose, Adam J. [VerfasserIn] |
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| Titel: | Phosphoproteomics-directed manipulation reveals SEC22B as a hepatocellular signaling node governing metabolic actions of glucagon |
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| Verf.angabe: | Yuqin Wu, Ashish Foollee, Andrea Y. Chan, Susanne Hille, Jana Hauke, Matthew P. Challis, Jared L. Johnson, Tomer M. Yaron, Victoria Mynard, Okka H. Aung, Maria Almira S. Cleofe, Cheng Huang, Terry C.C. Lim Kam Sian, Mohammad Rahbari, Suchira Gallage, Mathias Heikenwalder, Lewis C. Cantley, Ralf B. Schittenhelm, Luke E. Formosa, Greg C. Smith, Jürgen G. Okun, Oliver J. Müller, Patricia M. Rusu & Adam J. Rose |
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| E-Jahr: | 2024 |
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| Jahr: | 27 September 2024 |
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| Umfang: | 17 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | Gesehen am 12.05.2025 |
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| Titel Quelle: | Enthalten in: Nature Communications |
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| Ort Quelle: | [London] : Springer Nature, 2010 |
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| Jahr Quelle: | 2024 |
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| Band/Heft Quelle: | 15(2024), Artikel-ID 8390, Seite 1-17 |
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| ISSN Quelle: | 2041-1723 |
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| Abstract: | The peptide hormone glucagon is a fundamental metabolic regulator that is also being considered as a pharmacotherapeutic option for obesity and type 2 diabetes. Despite this, we know very little regarding how glucagon exerts its pleiotropic metabolic actions. Given that the liver is a chief site of action, we performed in situ time-resolved liver phosphoproteomics to reveal glucagon signaling nodes. Through pathway analysis of the thousands of phosphopeptides identified, we reveal “membrane trafficking” as a dominant signature with the vesicle trafficking protein SEC22 Homolog B (SEC22B) S137 phosphorylation being a top hit. Hepatocyte-specific loss- and gain-of-function experiments reveal that SEC22B was a key regulator of glycogen, lipid and amino acid metabolism, with SEC22B-S137 phosphorylation playing a major role in glucagon action. Mechanistically, we identify several protein binding partners of SEC22B affected by glucagon, some of which were differentially enriched with SEC22B-S137 phosphorylation. In summary, we demonstrate that phosphorylation of SEC22B is a hepatocellular signaling node mediating the metabolic actions of glucagon and provide a rich resource for future investigations on the biology of glucagon action. |
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| DOI: | doi:10.1038/s41467-024-52703-w |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1038/s41467-024-52703-w |
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| | kostenfrei: Volltext: https://www.nature.com/articles/s41467-024-52703-w |
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| | DOI: https://doi.org/10.1038/s41467-024-52703-w |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Cell signalling |
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| | Hormones |
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| | Membrane trafficking |
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| | Metabolism |
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| K10plus-PPN: | 1925311279 |
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| Verknüpfungen: | → Zeitschrift |
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Phosphoproteomics-directed manipulation reveals SEC22B as a hepatocellular signaling node governing metabolic actions of glucagon / Wu, Yuqin [VerfasserIn]; 27 September 2024 (Online-Ressource)
