| |  Online-Ressource |
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| Verfasst von: | Hohaus, Stefan [VerfasserIn]  |
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| | Pförsich, Margit [VerfasserIn] |
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| | Martin, Simona [VerfasserIn] |
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| | Abdallah Khalafallah, Alhossain [VerfasserIn] |
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| | Lin, Yung Sheng [VerfasserIn] |
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| | Funk, Liane [VerfasserIn] |
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| | Voso, Maria Teresa [VerfasserIn] |
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| | Kaul, Sepp [VerfasserIn] |
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| | Schmid, Hans [VerfasserIn] |
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| | Wallwiener, Diethelm [VerfasserIn] |
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| | Haas, Rainer [VerfasserIn] |
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| Titel: | Immunomagnetic selection of CD34+peripheral blood stem cells for autografting in patients with breast cancer |
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| Verf.angabe: | Stefan Hohaus, Margit Pförsich, Simona Murea, Alhossain Abdallah, Yung-Sheng Lin, Liane Funk, Maria Teresa Voso, Sepp Kaul, Hans Schmid, Diethelm Wallwiener, Rainer Haas |
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| Jahr: | 1997 |
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| Umfang: | 8 S. |
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| Fussnoten: | Elektronische Reproduktion der Druck-Ausgabe 29. Oktober 2003 ; Gesehen am 13.05.2025 |
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| Titel Quelle: | Enthalten in: British journal of haematology |
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| Ort Quelle: | Oxford [u.a.] : Wiley-Blackwell, 1955 |
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| Jahr Quelle: | 1997 |
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| Band/Heft Quelle: | 97(1997), 4, Seite 881-888 |
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| ISSN Quelle: | 1365-2141 |
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| Abstract: | Contamination of transplants with tumour cells may contribute to relapse after peripheral blood stem cell transplantation (PBSCT). We studied the feasibility of CD34+ cell selection from blood-derived autografts obtained following G-CSF-supported cytotoxic chemotherapy in a group of 25 patients with breast cancer (10 with high-risk stage II/III and 15 with stage IV without bone or bone marrow involvement). Using immunomagnetic beads (Isolex 300 SA, Baxter) CD34+ cells were enriched and released by chymopapain resulting in a median purity of 95% (range 82-99%) and a median recovery of 80% (range 27-132%). The enrichment procedure did not change the proportion of CD34+ subsets coexpressing HLA-DR, CD38 and Thy-1, while L-selectin was removed from the cell surface following selection. Using a sensitive immunocytological technique with a cocktail of epithelial-specific antibodies (anti-cytokeratin 8, 18 and 19; HEA125; BM7 and BM8), five leukaphereses products contained epithelial cells, whereas the selected CD34+ cell fraction was free of tumour cells. A neutrophil count of 0.5×109/l and a platelet count of 20×109/l was reached after a median time of 14 and 10 d following 40 high-dose chemotherapy (HDC) cycles. Our results indicate that immunomagnetic selection of CD34+ cells yields highly purified autografts devoid of tumour cells whereas the engraftment ability of the progenitor and stem cells is fully retained. |
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| DOI: | doi:10.1046/j.1365-2141.1997.1272941.x |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1046/j.1365-2141.1997.1272941.x |
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| | Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1046/j.1365-2141.1997.1272941.x |
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| | DOI: https://doi.org/10.1046/j.1365-2141.1997.1272941.x |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | autologous transplantation |
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| | breast cancer |
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| | CD34+ cells |
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| | positive selection |
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| K10plus-PPN: | 1925486524 |
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| Verknüpfungen: | → Zeitschrift |
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Immunomagnetic selection of CD34+peripheral blood stem cells for autografting in patients with breast cancer / Hohaus, Stefan [VerfasserIn]; 1997 (Online-Ressource)
