Synovial fibroblasts as target cells for staphylococcal enterotoxin-induced T-cell cytotoxicity

• Verfasst von: Kraft, Sabrina [VerfasserIn]
• Verfasst von: Filsinger, Suzanne [VerfasserIn]
• Verfasst von: Krämer, K.-L. [VerfasserIn]
• Verfasst von: Kabelitz, D. [VerfasserIn]
• Verfasst von: Hänsch, Gertrud Maria [VerfasserIn]
• Verfasst von: Schöls, Margarita [VerfasserIn]
• Titel: Synovial fibroblasts as target cells for staphylococcal enterotoxin-induced T-cell cytotoxicity
• Verf.angabe: M. Kraft, S. Filsinger, K.-L. Krämer, D. Kabelitz, G.M. Hänsch & M. Schoels
• E-Jahr: 1998
• Jahr: January 1998
• Umfang: 6 S.
• Fussnoten: Elektronische Reproduktion der Druck-Ausgabe 24. September 2008 ; Gesehen am 05.06.2025
• Titel Quelle: Enthalten in: Immunology
• Ort Quelle: Oxford [u.a.] : Wiley-Blackwell, 1958
• Jahr Quelle: 1998
• Band/Heft Quelle: 93(1998), 1, Seite 20-25
• ISSN Quelle: 1365-2567
• DOI: doi:10.1046/j.1365-2567.1998.00398.x
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1927581109

Abstract

Rheumatoid arthritis (RA) is a chronic autoimmune disease of unknown aetiology. Recently, superantigens have been implied in the pathogenesis of RA. Superantigens activate a large fraction of T cells leading to the production of cytokines and proliferation. In addition, superantigens direct cellular cytotoxicity towards major histocompatibility complex (MHC) class II-expressing cells. There is now increasing evidence that cytotoxic T cells may be involved in the pathogenesis of RA. In the inflamed synovia class II-positive synovial fibroblasts (SFC) are found. In the present study it was tested whether MHC class II-positive SFC serve as target cells for superantigen-induced cellular cytotoxicity. SFC were stimulated with interferon-γ to express class II antigens, then they were cultivated in the presence of CD4-positive T cells with or without staphylococcal enterotoxins (SE). Cytotoxicity of T cells was measured as release of lactate dehydrogenase from SFC. Specific cytotoxicity was only found in the presence of class II-positive SFC depending on the dose of SE. Maximum lysis was seen after 20 hr. T-cell cytotoxicity was inhibited by antibodies to MHC class II antigens. The data suggest that class II-positive SFC not only function as accessory cells for SE-mediated T-cell proliferation and interleukin-2 production but may also be the targets of superantigen-mediated cellular cytotoxicity.