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Status: Bibliographieeintrag

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Verfasst von:Hartley, Anna [VerfasserIn]   i
 Burger, Luisa [VerfasserIn]   i
 Wincek, Cornelia L. [VerfasserIn]   i
 Dons, Lieke [VerfasserIn]   i
 Li, Tracy [VerfasserIn]   i
 Grewenig, Annabel [VerfasserIn]   i
 Tasgin, Toros [VerfasserIn]   i
 Urban, Manuela Vanessa [VerfasserIn]   i
 Roig-Merino, Alicia [VerfasserIn]   i
 Ghazvini, Mehrnaz [VerfasserIn]   i
 Harbottle, Richard P. [VerfasserIn]   i
Titel:A simple nonviral method to generate human induced pluripotent stem cells using SMAR DNA vectors
Verf.angabe:Anna Hartley, Luisa Burger, Cornelia L. Wincek, Lieke Dons, Tracy Li, Annabel Grewenig, Toros Taşgın, Manuela Urban, Alicia Roig-Merino, Mehrnaz Ghazvini and Richard P. Harbottle
E-Jahr:2024
Jahr:30 April 2024
Umfang:17 S.
Illustrationen:Illustrationen
Fussnoten:Gesehen am 11.06.2025
Titel Quelle:Enthalten in: Genes
Ort Quelle:Basel : MDPI, 2009
Jahr Quelle:2024
Band/Heft Quelle:15(2024), 5, Artikel-ID 575, Seite 1-17
ISSN Quelle:2073-4425
Abstract:Induced pluripotent stem cells (iPSCs) are a powerful tool for biomedical research, but their production presents challenges and safety concerns. Yamanaka and Takahashi revolutionised the field by demonstrating that somatic cells could be reprogrammed into pluripotent cells by overexpressing four key factors for a sufficient time. iPSCs are typically generated using viruses or virus-based methods, which have drawbacks such as vector persistence, risk of insertional mutagenesis, and oncogenesis. The application of less harmful nonviral vectors is limited as conventional plasmids cannot deliver the levels or duration of the factors necessary from a single transfection. Hence, plasmids that are most often used for reprogramming employ the potentially oncogenic Epstein-Barr nuclear antigen 1 (EBNA-1) system to ensure adequate levels and persistence of expression. In this study, we explored the use of nonviral SMAR DNA vectors to reprogram human fibroblasts into iPSCs. We show for the first time that iPSCs can be generated using nonviral plasmids without the use of EBNA-1 and that these DNA vectors can provide sufficient expression to induce pluripotency. We describe an optimised reprogramming protocol using these vectors that can produce high-quality iPSCs with comparable pluripotency and cellular function to those generated with viruses or EBNA-1 vectors.
DOI:doi:10.3390/genes15050575
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

kostenfrei: Volltext: https://doi.org/10.3390/genes15050575
 kostenfrei: Volltext: https://www.mdpi.com/2073-4425/15/5/575
 DOI: https://doi.org/10.3390/genes15050575
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Cells, Cultured
 Cellular Reprogramming
 Epstein-Barr Virus Nuclear Antigens
 Fibroblasts
 Genetic Vectors
 Humans
 Induced Pluripotent Stem Cells
 iPSC
 nonviral
 Plasmids
 reprogramming
 S/MAR
 SMAR DNA vector
 stem cells
 Transfection
K10plus-PPN:1927943345
Verknüpfungen:→ Zeitschrift

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