Establishing an auxin-inducible GFP nanobody-based acute protein knockdown system to mimic hypomorphic mutations during early medaka embryogenesis

• Verfasst von: Pakari, Kaisa [VerfasserIn]
• Verfasst von: Jakab, Sevinç [VerfasserIn]
• Verfasst von: Sánchez Salvador, Encarnación [VerfasserIn]
• Verfasst von: Thiel, Christian [VerfasserIn]
• Verfasst von: Wittbrodt, Joachim [VerfasserIn]
• Verfasst von: Thumberger, Thomas [VerfasserIn]
• Titel: Establishing an auxin-inducible GFP nanobody-based acute protein knockdown system to mimic hypomorphic mutations during early medaka embryogenesis
• Verf.angabe: Kaisa Pakari, Sevinç Jakab, Encarnación Sánchez Salvador, Christian Thiel, Joachim Wittbrodt, Thomas Thumberger
• E-Jahr: 2025
• Jahr: May 23, 2025
• Umfang: 22 S.
• Fussnoten: Gesehen am 11.06.2025
• Titel Quelle: Enthalten in: bioRxiv beta
• Ort Quelle: Cold Spring Harbor : Cold Spring Harbor Laboratory, NY, 2013
• Jahr Quelle: 2025
• Band/Heft Quelle: (2025) vom: Mai, Artikel-ID 2025.05.23.655727, Seite 1-22
• DOI: doi:10.1101/2025.05.23.655727
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1927954088

Abstract

Creating hypomorphic mutations are crucial to study gene function in vivo, especially when null mutations result in (embryonic) lethality. This is especially the case for enzymes involved in glycosylation that, when mutated in human patients, are causing the disease congenital disorders of glycosylation (CDG). To resemble the patient conditions, it would be ideal to acutely modulate the proteins in question to directly interfere with protein levels of such essential enzymes. These methods offer to establish pathogenic enzyme levels resembling net enzyme activity reported from patients suffering from CDG, with Phosphomannomutase 2 - CDG (PMM2-CDG) as the most common form. We established an auxin-inducible acute protein knockdown system for the use in the teleost fish medaka (Oryzias latipes) by combining an improved degron (AID2) technology with a mAID-nanobody targeting endogenously GFP-tagged Pmm2 protein. We generated a fishline expressing a functional Pmm2-GFP fusion protein, by single copy integration of GFP into the pmm2 locus. Upon induction, the degron system efficiently reduced Pmm2-GFP levels and enzyme activity, recapitulating the activity level of the hypomorphic mutations associated with PMM2-CDG in patients. This broadly applicable approach enables the investigation of CDG disease mechanisms during early embryonic development through reduction of protein abundance mimicking hypomorphic mutations and thus substantially expands the range of the genetic toolbox.