| |  Online-Ressource |
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| Verfasst von: | Denzer, Lea [VerfasserIn]  |
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| | Muranyi, Walter [VerfasserIn] |
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| | Herold, Rosanna [VerfasserIn] |
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| | Stump-Guthier, Carolin [VerfasserIn] |
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| | Ishikawa, Hiroshi [VerfasserIn] |
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| | Sticht, Carsten [VerfasserIn] |
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| | Schroten, Horst [VerfasserIn] |
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| | Schwerk, Christian [VerfasserIn] |
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| | Weichert, Stefan [VerfasserIn] |
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| Titel: | Transcriptome and functional comparison of primary and immortalized endothelial cells of the human choroid plexus at the blood-cerebrospinal fluid barrier |
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| Verf.angabe: | Lea Denzer, Walter Muranyi, Rosanna Herold, Carolin Stump-Guthier, Hiroshi Ishikawa, Carsten Sticht, Horst Schroten, Christian Schwerk and Stefan Weichert |
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| E-Jahr: | 2025 |
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| Jahr: | 19 February 2025 |
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| Umfang: | 21 S. |
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| Illustrationen: | Illustrationen, Diagramme |
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| Fussnoten: | Gesehen am 08.07.2025 |
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| Weitere Titel: | Titel des übergeordneten Special issue: Advanced research progress of blood-brain barrier |
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| Titel Quelle: | Enthalten in: International journal of molecular sciences |
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| Ort Quelle: | Basel : Molecular Diversity Preservation International, 2000 |
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| Jahr Quelle: | 2025 |
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| Band/Heft Quelle: | 26(2025), 4, Artikel-ID 1779, Seite 1-21 |
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| ISSN Quelle: | 1422-0067 |
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| | 1661-6596 |
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| Abstract: | The human choroid plexus (CP) is the location of the blood-cerebrospinal fluid (CSF) barrier (BCSFB). Whereas the epithelial cells of the CP mainly contribute to the formation of the BCSFB, the vessels of the CP are built by fenestrated endothelial cells. Still, the CP endothelium can contribute to barrier function. By ectopic expression of human telomerase reverse transcriptase (hTERT) in primary human CP endothelial cells (HCPEnCs), we recently generated and characterized immortalized HCPEnCs (iHCPEnCs). Here, we compared primary cells of the sixth passage (HCPEnCs p6) with a lower (p20) and a higher passage (p50) of iHCPEnCs by transcriptome analysis. A high concordance of HCPEnCs and both passages of iHCPEnCs was observed, as only small proportions of the transcripts examined were significantly altered. Differentially expressed genes (DEGs) were identified and assigned to potentially affected biological processes by gene set enrichment analysis (GSEA). Various components of the endothelial barrier-relevant Wnt signaling were detected in HCPEnCs and iHCPEnCs. Functional analysis of HCPEnCs and iHCPEnCs showed equal marginal activation of Wnt signaling, supporting the downregulation of β-catenin (CTNNB) signaling in CP endothelial cells, and a contribution to the barrier function by the CP endothelium was retained until passage 100 (p100) of iHCPEnCs. Overall, our data support the suitability of iHCPEnCs as an in vitro model of the CP endothelium over extended passages. |
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| DOI: | doi:10.3390/ijms26041779 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.3390/ijms26041779 |
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| | kostenfrei: Volltext: https://www.mdpi.com/1422-0067/26/4/1779 |
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| | DOI: https://doi.org/10.3390/ijms26041779 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | blood-cerebrospinal fluid barrier |
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| | choroid plexus |
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| | endothelium |
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| | transcriptome |
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| | Wnt signaling |
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| K10plus-PPN: | 1929948352 |
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| Verknüpfungen: | → Zeitschrift |
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Transcriptome and functional comparison of primary and immortalized endothelial cells of the human choroid plexus at the blood-cerebrospinal fluid barrier / Denzer, Lea [VerfasserIn]; 19 February 2025 (Online-Ressource)
