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| Verfasst von: | Grand, Ralph Stefan [VerfasserIn]  |
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| | Pregnolato, Marco [VerfasserIn] |
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| | Baumgartner, Lisa [VerfasserIn] |
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| | Hoerner, Leslie [VerfasserIn] |
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| | Burger, Lukas [VerfasserIn] |
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| | Schübeler, Dirk [VerfasserIn] |
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| Titel: | Genome access is transcription factor-specific and defined by nucleosome position |
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| Verf.angabe: | Ralph Stefan Grand, Marco Pregnolato, Lisa Baumgartner, Leslie Hoerner, Lukas Burger and Dirk Schübeler |
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| E-Jahr: | 2024 |
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| Jahr: | September 19, 2024 |
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| Umfang: | 14 S. |
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| Fussnoten: | Gesehen am 09.07.2025 |
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| Titel Quelle: | Enthalten in: Molecular cell |
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| Ort Quelle: | [Cambridge, Mass.] : Cell Press, 1997 |
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| Jahr Quelle: | 2024 |
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| Band/Heft Quelle: | 84(2024), 18, Seite 3455-3468 |
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| ISSN Quelle: | 1097-4164 |
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| Abstract: | Mammalian gene expression is controlled by transcription factors (TFs) that engage sequence motifs in a chromatinized genome, where nucleosomes can restrict DNA access. Yet, how nucleosomes affect individual TFs remains unclear. Here, we measure the ability of over one hundred TF motifs to recruit TFs in a defined chromosomal locus in mouse embryonic stem cells. This identifies a set sufficient to enable the binding of TFs with diverse tissue specificities, functions, and DNA-binding domains. These chromatin-competent factors are further classified when challenged to engage motifs within a highly phased nucleosome. The pluripotency factors OCT4-SOX2 preferentially engage non-nucleosomal and entry-exit motifs, but not nucleosome-internal sites, a preference that also guides binding genome wide. By contrast, factors such as BANP, REST, or CTCF engage throughout, causing nucleosomal displacement. This supports that TFs vary widely in their sensitivity to nucleosomes and that genome access is TF specific and influenced by nucleosome position in the cell. |
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| DOI: | doi:10.1016/j.molcel.2024.08.009 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1016/j.molcel.2024.08.009 |
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| | kostenfrei: Volltext: https://www.sciencedirect.com/science/article/pii/S1097276524006634 |
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| | DOI: https://doi.org/10.1016/j.molcel.2024.08.009 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | accessibility |
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| | chromatin |
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| | genome |
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| | nucleosome |
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| | pioneer factors |
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| | transcription factors |
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| K10plus-PPN: | 1930051670 |
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| Verknüpfungen: | → Zeitschrift |
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Genome access is transcription factor-specific and defined by nucleosome position / Grand, Ralph Stefan [VerfasserIn]; September 19, 2024 (Online-Ressource)
