| |  Online-Ressource |
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| Verfasst von: | Semerci Sevimli, Tuğba [VerfasserIn]  |
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| | Inan, Ulukan [VerfasserIn] |
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| | Qomi Ekenel, Emilia [VerfasserIn] |
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| | Ozgul, Cemre [VerfasserIn] |
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| | Danaci, Cem Ozgur [VerfasserIn] |
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| | Cetinkaya, Sevval [VerfasserIn] |
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| | Ahmadova, Zarifa [VerfasserIn] |
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| Titel: | Synovial fluid mesenchymal stem cell-derived microRNA-127-5p can modulate transforming growth factor-β signaling after in vitro chondrogenic induction |
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| Verf.angabe: | Tugba Semerci Sevimli, Ulukan Inan, Emilia Qomi Ekenel, Cemre Ozgul, Cem Ozgur Danaci, Sevval Cetinkaya, Zarifa Ahmadova |
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| E-Jahr: | 2025 |
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| Jahr: | February 2025 |
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| Umfang: | 12 S. |
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| Illustrationen: | Illustrationen, Diagramme |
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| Fussnoten: | Online veröffentlicht: 27. November 2024 ; Gesehen am 22.07.2025 |
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| Titel Quelle: | Enthalten in: Cytotechnology |
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| Ort Quelle: | Dordrecht [u.a.] : Springer Science + Business Media B.V., 1987 |
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| Jahr Quelle: | 2025 |
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| Band/Heft Quelle: | 77(2025), 1 vom: Feb., Artikel-ID 8, Seite 1-12 |
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| ISSN Quelle: | 1573-0778 |
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| Abstract: | MicroRNA profiling in human cartilage is necessary for chondrogenesis. The study aimed to compare microRNA 127-5p (miR-127-5p) and TGF-β signaling pathway gene expressions of human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) and synovial fluid-derived stem cells (hSF-MSCs) after induced chondrogenesis. MSCs induced into chondrogenic differentiation. Alcian Blue and Safranin O staining were performed to determine chondrogenic differentiation. The RT-qPCR determined the expression levels of miR-127-5p and TGF-β signaling pathway genes. miR-127-5p expression was significantly higher in chondrogenic differentiated hSF-MSCs (dhSF-MSCs) (p < 0.05). TGF-β, SMAD2, and SMAD3 expressions were substantially higher in dhSF-MSCs (all p < 0.001), while SMAD4, and ACAN expressions were downregulated (all p < 0.001). No difference was detected between COL1A2 expression levels. This study suggests that miR-127-5p derived from hSF-MSCs may regulate chondrogenesis, thereby inducing the TGF-β pathway activation, and also presents, for the first time, a comparative analysis of the expression of miR-127-5p and the TGF-β signaling pathway genes of hSF-MSCs and hAT-MSCs concerning differences in chondrogenic potential. Graphical abstract |
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| DOI: | doi:10.1007/s10616-024-00660-z |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1007/s10616-024-00660-z |
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| | Volltext: http://link.springer.com/article/10.1007/s10616-024-00660-z |
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| | DOI: https://doi.org/10.1007/s10616-024-00660-z |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 193142456X |
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| Verknüpfungen: | → Zeitschrift |
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Synovial fluid mesenchymal stem cell-derived microRNA-127-5p can modulate transforming growth factor-β signaling after in vitro chondrogenic induction / Semerci Sevimli, Tuğba [VerfasserIn]; February 2025 (Online-Ressource)
