Titel: | Immunoelectron microscopy |
Titelzusatz: | methods and protocols |
Mitwirkende: | Schwartzbach, Steven D. [Hrsg.]  |
| Osafune, Tetsuaki  |
Verf.angabe: | Ed. by Steven D. Schwartzbach; Tetsuaki Osafune |
Verlagsort: | New York ; Heidelberg [u.a.] |
Verlag: | Humana Press |
Jahr: | 2010 |
Umfang: | XI, 352 S. |
Illustrationen: | Ill. |
Gesamttitel/Reihe: | Methods in molecular biology ; 657 |
| Springer protocols |
Inhalt: | Protein antigen expression in escherichia coli for antibody production / D.M. Rancour, S.K. Backues and S.Y. Bednarek |
| Expression of epitope-tagged proteins in plants / T. Furuichi |
| Expression of epitope-tagged proteins in arabidopsis leaf mesophyll protoplasts / Y.H. Cho and S.D. Yoo |
| Transient expression of epitope-tagged proteins in mammalian cells / M.L. Styers, J. Lowery and E. Sztul |
| Production and purification of polyclonal antibodies / M. Nakazawa, M. Mukumoto and K. Miyatake |
| Production and purification of monoclonal antibodies / M. Nakazawa, M. Mukumoto and K. Miyatake |
| Production of antipeptide antibodies / B.S. Lee ... [et al.] |
| Preparation of colloidal gold particles and conjugation to protein A, IgG, F(ab')₂, and streptavidin / S. Yokota |
| Immunoelectron microscopy of chemically fixed developing plant embryos / T. Osafune and S.D. Schwartzbach |
| Pre-embedding immunogold localization of antigens in mammalian brain slices / T. Schikorski |
| Pre-embedding immunoelectron microscopy of chemically fixed mammalian tissue culture cells / H. Hagiwara ... [et al.] |
| Immunoelectron microscopy of cryofixed and freeze-substituted plant tissues / M. Takeuchi, K. Takabe and Y. Mineyuki |
| In vivo cryotechniques for preparation of animal tissues for immunoelectron microscopy / S. Ohno ... [et al.] |
| Immunoelectron microscopy of cryofixed freeze-substituted mammalian tissue culture cells / A. Sawaguchi |
| Immunoelectron microscopy of cryofixed freeze-substituted saccharomyces cerevisiae / J. Fiserova and M.W. Goldberg |
| High-resolution molecular localization by freeze-fracture replica labeling / A. Fujita and T. Fujimoto |
| Pre-embedding electron microscopy methods for glycan localization in chemically fixed mammalian tissue using horseradish peroxidase-conjugated lectin / Y. Akimoto and H. Kawakami |
| Pre-embedding nanogold silver and gold intensification / A. Yamamoto and R. Masaki |
| The post-embedding method for immunoelectron microscopy of mammalian tissues: A standardized procedure based on heat-induced antigen retrieval / S. Yamashita |
| Double-label immunoelectron microscopy for studying the colocalization of proteins in cultured cells / H. Hagiwara ... [et al.] |
| Serial section immunoelectron microscopy of algal cells / T. Osafune and S.D. Schwartzbach |
| Freeze-etch electron tomography for the plasma membrane interface / N. Morone |
| Localization of rDNA at nucleolar structural components by immunoelectron microscopy / S. Sato and Y. Sato |
| Immunogold labelling for scanning electron microscopy / M.W. Goldberg and J. Fiserova |
| Horseradish peroxidase as a reporter gene and as a cell-organelle-specific marker in correlative light-electron microscopy / T. Schikorski |
| Monitoring rapid endocytosis in the electron microscope via photoconversion of vesicles fluorescently labeled with FM1-43 / T. Schikorski. |
ISBN: | 978-1-60761-782-2 |
Bestellnr.: | 12221103 |
URL: | Inhaltstext: http://deposit.d-nb.de/cgi-bin/dokserv?id=3442350&prov=M&dok_var=1&dok_ext=htm |
| Cover: https://swbplus.bsz-bw.de/bsz322991021cov.jpg |
Schlagwörter: | (s)Elektronenmikroskopie / (s)Biomolekül / (s)Markierung <Chemie> / (s)Antikörper / (s)Epitop / (s)Methode  |
| (s)Immunelektronenmikroskopie / (s)Methode  |
| (s)Immunelektronenmikroskopie / (s)Methode  |
Dokumenttyp: | Aufsatzsammlung |
Sprache: | eng |
Reproduktion: | Online-Ausg.: Schwartzbach, Steven D.: Immunoelectron Microscopy. - Totowa, NJ: Humana Press, 2010. - Online-Ressource (XI, 352p, digital) |
Sonstige Nr.: | Best.-Nr.: 12221103 |
RVK-Notation: | WC 3100  |
K10plus-PPN: | 622496174 |
Verknüpfungen: | → Übergeordnete Aufnahme |
Immunoelectron microscopy / Schwartzbach, Steven D. [Hrsg.]; 2010