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Standort: Zweigstelle Neuenheim / Freihandbereich Monograph  3D-Plan
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Titel:Immunoelectron microscopy
Titelzusatz:methods and protocols
Mitwirkende:Schwartzbach, Steven D. [Hrsg.]   i
 Osafune, Tetsuaki   i
Verf.angabe:Ed. by Steven D. Schwartzbach; Tetsuaki Osafune
Verlagsort:New York ; Heidelberg [u.a.]
Verlag:Humana Press
Jahr:2010
Umfang:XI, 352 S.
Illustrationen:Ill.
Gesamttitel/Reihe:Methods in molecular biology ; 657
 Springer protocols
Inhalt:Protein antigen expression in escherichia coli for antibody production / D.M. Rancour, S.K. Backues and S.Y. Bednarek
 Expression of epitope-tagged proteins in plants / T. Furuichi
 Expression of epitope-tagged proteins in arabidopsis leaf mesophyll protoplasts / Y.H. Cho and S.D. Yoo
 Transient expression of epitope-tagged proteins in mammalian cells / M.L. Styers, J. Lowery and E. Sztul
 Production and purification of polyclonal antibodies / M. Nakazawa, M. Mukumoto and K. Miyatake
 Production and purification of monoclonal antibodies / M. Nakazawa, M. Mukumoto and K. Miyatake
 Production of antipeptide antibodies / B.S. Lee ... [et al.]
 Preparation of colloidal gold particles and conjugation to protein A, IgG, F(ab')₂, and streptavidin / S. Yokota
 Immunoelectron microscopy of chemically fixed developing plant embryos / T. Osafune and S.D. Schwartzbach
 Pre-embedding immunogold localization of antigens in mammalian brain slices / T. Schikorski
 Pre-embedding immunoelectron microscopy of chemically fixed mammalian tissue culture cells / H. Hagiwara ... [et al.]
 Immunoelectron microscopy of cryofixed and freeze-substituted plant tissues / M. Takeuchi, K. Takabe and Y. Mineyuki
 In vivo cryotechniques for preparation of animal tissues for immunoelectron microscopy / S. Ohno ... [et al.]
 Immunoelectron microscopy of cryofixed freeze-substituted mammalian tissue culture cells / A. Sawaguchi
 Immunoelectron microscopy of cryofixed freeze-substituted saccharomyces cerevisiae / J. Fiserova and M.W. Goldberg
 High-resolution molecular localization by freeze-fracture replica labeling / A. Fujita and T. Fujimoto
 Pre-embedding electron microscopy methods for glycan localization in chemically fixed mammalian tissue using horseradish peroxidase-conjugated lectin / Y. Akimoto and H. Kawakami
 Pre-embedding nanogold silver and gold intensification / A. Yamamoto and R. Masaki
 The post-embedding method for immunoelectron microscopy of mammalian tissues: A standardized procedure based on heat-induced antigen retrieval / S. Yamashita
 Double-label immunoelectron microscopy for studying the colocalization of proteins in cultured cells / H. Hagiwara ... [et al.]
 Serial section immunoelectron microscopy of algal cells / T. Osafune and S.D. Schwartzbach
 Freeze-etch electron tomography for the plasma membrane interface / N. Morone
 Localization of rDNA at nucleolar structural components by immunoelectron microscopy / S. Sato and Y. Sato
 Immunogold labelling for scanning electron microscopy / M.W. Goldberg and J. Fiserova
 Horseradish peroxidase as a reporter gene and as a cell-organelle-specific marker in correlative light-electron microscopy / T. Schikorski
 Monitoring rapid endocytosis in the electron microscope via photoconversion of vesicles fluorescently labeled with FM1-43 / T. Schikorski.
ISBN:978-1-60761-782-2
Bestellnr.:12221103
URL:: Inhaltstext: http://deposit.d-nb.de/cgi-bin/dokserv?id=3442350&prov=M&dok_var=1&dok_ext=htm
 : Cover ; Verlag: http://swbplus.bsz-bw.de/bsz322991021cov.htm
 : Inhaltstext: http://deposit.d-nb.de/cgi-bin/dokserv?id=3442350&prov=M&dok_var=1&dok_ext=htm
Schlagwörter:(s)Elektronenmikroskopie   i / (s)Biomolekül   i / (s)Markierung <Chemie>   i / (s)Antikörper   i / (s)Epitop   i / (s)Methode   i
 (s)Immunelektronenmikroskopie   i / (s)Methode   i
Dokumenttyp:Aufsatzsammlung
Sprache:eng
Reproduktion:Online-Ausg.: Schwartzbach, Steven D.: Immunoelectron Microscopy. - Totowa, NJ: Humana Press, 2010. - Online-Ressource (XI, 352p, digital)
Sonstige Nr.:Best.-Nr.: 12221103
RVK-Notation:WC 3100   i
K10plus-PPN:622496174
Verknüpfungen:→ Übergeordnete Aufnahme
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