Live cell imaging with R-GECO1 sheds light on flg22- and Chitin-induced transient [Ca2+]cyt patterns in Arabidopsis

• Verfasst von: Keinath, Nana [VerfasserIn]
• Verfasst von: Waadt, Rainer [VerfasserIn]
• Verfasst von: Brugman, Rik [VerfasserIn]
• Verfasst von: Großmann, Guido [VerfasserIn]
• Verfasst von: Schumacher, Karin [VerfasserIn]
• Verfasst von: Krebs, Melanie [VerfasserIn]
• Titel: Live cell imaging with R-GECO1 sheds light on flg22- and Chitin-induced transient [Ca2+]cyt patterns in Arabidopsis
• Verf.angabe: Nana F. Keinath, Rainer Waadt, Rik Brugman, Julian I. Schroeder, Guido Grossmann, Karin Schumacher, Melanie Krebs
• E-Jahr: 2015
• Jahr: 19 May 2015
• Umfang: 13 S.
• Fussnoten: Gesehen am 07.06.2017
• Titel Quelle: Enthalten in: Molecular plant
• Ort Quelle: [Cambridge, MA] : Cell Press, 2008
• Jahr Quelle: 2015
• Band/Heft Quelle: 8(2015), 8, Seite 1188-1200
• ISSN Quelle: 1752-9867
• DOI: doi:10.1016/j.molp.2015.05.006
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1559503106

Abstract

Intracellular Ca2+ transients are an integral part of the signaling cascade during pathogen-associated molecular pattern (PAMP)-triggered immunity in plants. Yet, our knowledge about the spatial distribution of PAMP-induced Ca2+ signals is limited. Investigation of cell- and tissue-specific properties of Ca2+-dependent signaling processes requires versatile Ca2+ reporters that are able to extract spatial information from cellular and subcellular structures, as well as from whole tissues over time periods from seconds to hours. Fluorescence-based reporters cover both a broad spatial and temporal range, which makes them ideally suited to study Ca2+ signaling in living cells. In this study, we compared two fluorescence-based Ca2+ sensors: the Förster resonance energy transfer (FRET)-based reporter yellow cameleon NES-YC3.6 and the intensity-based sensor R-GECO1. We demonstrate that R-GECO1 exhibits a significantly increased signal change compared with ratiometric NES-YC3.6 in response to several stimuli. Due to its superior sensitivity, R-GECO1 is able to report flg22- and chitin-induced Ca2+ signals on a cellular scale, which allowed identification of defined [Ca2+]cyt oscillations in epidermal and guard cells in response to the fungal elicitor chitin. Moreover, we discovered that flg22- and chitin-induced Ca2+ signals in the root initiate from the elongation zone.