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| Online-Ressource |
Verfasst von: | Boffi, Juan C. [VerfasserIn]  |
| Knabbe, Johannes [VerfasserIn]  |
| Kaiser, Michaela [VerfasserIn]  |
| Kuner, Thomas [VerfasserIn]  |
Titel: | KCC2-dependent steady-state intracellular chloride concentration and pH in cortical layer 2/3 neurons of anesthetized and awake mice |
Verf.angabe: | Juan C. Boffi, Johannes Knabbe, Michaela Kaiser and Thomas Kuner |
E-Jahr: | 2018 |
Jahr: | 25 January 2018 |
Fussnoten: | Gesehen am 13.04.2018 |
Titel Quelle: | Enthalten in: Frontiers in cellular neuroscience |
Ort Quelle: | Lausanne : Frontiers Research Foundation, 2007 |
Jahr Quelle: | 2018 |
Band/Heft Quelle: | 12(2018) Artikel-Nummer 7, 14 Seiten |
ISSN Quelle: | 1662-5102 |
Abstract: | Neuronal intracellular Cl- concentration ([Cl-]i) influences a wide range of processes such as neuronal inhibition, membrane potential dynamics, intracellular pH (pHi) or cell volume. Up to date, neuronal [Cl-]i has predominantly been studied in model systems of reduced complexity. Here, we implemented the genetically encoded ratiometric Cl- indicator Superclomeleon (SCLM) to estimate the steady-state [Cl-]i in cortical neurons from anesthetized and awake mice using 2-photon microscopy. Additionally, we implemented superecliptic pHluorin as a ratiometric sensor to estimate the intracellular steady-state pH (pHi) of mouse cortical neurons in vivo. We estimated an average resting [Cl-]i of 6 ± 2 mM with no evidence of subcellular gradients in the proximal somato-dendritic domain and an average somatic pHi of 7.1 ± 0.2. Neither [Cl-]i nor pHi were affected by isoflurane anesthesia. We deleted the cation-Cl- co-transporter KCC2 in single identified neurons of adult mice and found an increase of [Cl-]i to approximately 26 ± 8 mM, demonstrating that under in vivo conditions KCC2 produces low [Cl-]i in adult mouse neurons. In summary, neurons of the brain of awake adult mice exhibit a low and evenly distributed [Cl-]i in the proximal somato-dendritic compartment that is independent of anesthesia and requires KCC2 expression for its maintenance. |
DOI: | doi:10.3389/fncel.2018.00007 |
URL: | Kostenfrei: Volltext ; Verlag: http://dx.doi.org/10.3389/fncel.2018.00007 |
| Kostenfrei: Volltext: https://www.frontiersin.org/articles/10.3389/fncel.2018.00007/full |
| DOI: https://doi.org/10.3389/fncel.2018.00007 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | chloride |
| in vivo |
| intracellular |
| neuronal |
| pH |
| ratiometric imaging. |
K10plus-PPN: | 157200553X |
Verknüpfungen: | → Zeitschrift |
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Lokale URL UB: | Zum Volltext |
KCC2-dependent steady-state intracellular chloride concentration and pH in cortical layer 2/3 neurons of anesthetized and awake mice / Boffi, Juan C. [VerfasserIn]; 25 January 2018 (Online-Ressource)
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