Navigation überspringen
Universitätsbibliothek Heidelberg
Standort: ---
Exemplare: ---
heiBIB
 Online-Ressource
Verfasst von:Boffi, Juan C. [VerfasserIn]   i
 Knabbe, Johannes [VerfasserIn]   i
 Kaiser, Michaela [VerfasserIn]   i
 Kuner, Thomas [VerfasserIn]   i
Titel:KCC2-dependent steady-state intracellular chloride concentration and pH in cortical layer 2/3 neurons of anesthetized and awake mice
Verf.angabe:Juan C. Boffi, Johannes Knabbe, Michaela Kaiser and Thomas Kuner
E-Jahr:2018
Jahr:25 January 2018
Fussnoten:Gesehen am 13.04.2018
Titel Quelle:Enthalten in: Frontiers in cellular neuroscience
Ort Quelle:Lausanne : Frontiers Research Foundation, 2007
Jahr Quelle:2018
Band/Heft Quelle:12(2018) Artikel-Nummer 7, 14 Seiten
ISSN Quelle:1662-5102
Abstract:Neuronal intracellular Cl- concentration ([Cl-]i) influences a wide range of processes such as neuronal inhibition, membrane potential dynamics, intracellular pH (pHi) or cell volume. Up to date, neuronal [Cl-]i has predominantly been studied in model systems of reduced complexity. Here, we implemented the genetically encoded ratiometric Cl- indicator Superclomeleon (SCLM) to estimate the steady-state [Cl-]i in cortical neurons from anesthetized and awake mice using 2-photon microscopy. Additionally, we implemented superecliptic pHluorin as a ratiometric sensor to estimate the intracellular steady-state pH (pHi) of mouse cortical neurons in vivo. We estimated an average resting [Cl-]i of 6 ± 2 mM with no evidence of subcellular gradients in the proximal somato-dendritic domain and an average somatic pHi of 7.1 ± 0.2. Neither [Cl-]i nor pHi were affected by isoflurane anesthesia. We deleted the cation-Cl- co-transporter KCC2 in single identified neurons of adult mice and found an increase of [Cl-]i to approximately 26 ± 8 mM, demonstrating that under in vivo conditions KCC2 produces low [Cl-]i in adult mouse neurons. In summary, neurons of the brain of awake adult mice exhibit a low and evenly distributed [Cl-]i in the proximal somato-dendritic compartment that is independent of anesthesia and requires KCC2 expression for its maintenance.
DOI:doi:10.3389/fncel.2018.00007
URL:Kostenfrei: Volltext ; Verlag: http://dx.doi.org/10.3389/fncel.2018.00007
 Kostenfrei: Volltext: https://www.frontiersin.org/articles/10.3389/fncel.2018.00007/full
 DOI: https://doi.org/10.3389/fncel.2018.00007
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:chloride
 in vivo
 intracellular
 neuronal
 pH
 ratiometric imaging.
K10plus-PPN:157200553X
Verknüpfungen:→ Zeitschrift
 
 
Lokale URL UB: Zum Volltext

Permanenter Link auf diesen Titel (bookmarkfähig):  https://katalog.ub.uni-heidelberg.de/titel/68242624   QR-Code
zum Seitenanfang