| Online-Ressource |
Verfasst von: | Schüle-Freyer, Rebecca [VerfasserIn]  |
| Dictus, Christine [VerfasserIn]  |
| Campos, Benito [VerfasserIn]  |
| Wan, Feng [VerfasserIn]  |
| Ahmadi, Rezvan [VerfasserIn]  |
| Centner, Franz-Simon [VerfasserIn]  |
| Grabe, Niels [VerfasserIn]  |
| Lorenzo Bermejo, Justo [VerfasserIn]  |
| Unterberg, Andreas [VerfasserIn]  |
| Herold-Mende, Christel [VerfasserIn]  |
Titel: | Potential canonical wnt pathway activation in high-grade astrocytomas |
Verf.angabe: | Rebecca Schüle, Christine Dictus, Benito Campos, Feng Wan, Jörg Felsberg, Rezvan Ahmadi, Franz-Simon Centner, Niels Grabe, Guido Reifenberger, Justo L. Bermejo, Andreas Unterberg, and Christel Herold-Mende |
E-Jahr: | 2012 |
Jahr: | 2 May 2012 |
Umfang: | 11 S. |
Fussnoten: | Gesehen am 13.04.2018 |
Titel Quelle: | Enthalten in: The ScientificWorld journal |
Ort Quelle: | Boynton Beach, Fla. : [Verlag nicht ermittelbar], 2000 |
Jahr Quelle: | 2012 |
Band/Heft Quelle: | (2012), Artikel-ID 697313, Seite 1-11 |
ISSN Quelle: | 1537-744X |
Abstract: | Aberrant wnt pathway activation through cytoplasmic stabilization of β-catenin is crucial for the development of various human malignancies. In gliomagenesis, the role of canonical (i.e., β-catenin-dependent) signalling is largely unknown. Here, we studied canonical wnt pathway activation in 15 short-term cultures from high-grade gliomas and potential pathomechanisms leading to cytoplasmic β-catenin accumulation. Furthermore, we assessed the prognostic relevance of β-catenin expression in a tissue microarray comprising 283 astrocytomas. Expression of β-catenin, its transcriptional cofactors TCF-1 and TCF-4 as well as GSK-3β and APC, constituents of the β-catenin degradation complex was confirmed by RT-PCR in all cultures. A cytoplasmic β-catenin pool was detectable in 13/15 cultures leading to some transcriptional activity assessed by luciferase reporter gene assay in 8/13. Unlike other malignancies, characteristic mutations of β-catenin and APC leading to cytoplasmic stabilization of β-catenin were excluded by direct sequencing or protein truncation test. In patient tissues, β-catenin expression was directly and its degradation product's (β-catenin-P654) expression was inversely correlated with WHO grade. Increased β-catenin expression and low β-catenin-P654 expression were associated with shorter survival. Altogether, we report on potential canonical wnt pathway activation in high-grade gliomas and demonstrate that β-catenin expression in astrocytomas is associated with increased malignancy and adverse outcome. |
DOI: | doi:10.1100/2012/697313 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1100/2012/697313 |
| kostenfrei: Volltext: https://onlinelibrary.wiley.com/doi/full/10.1100/2012/697313 |
| DOI: https://doi.org/10.1100/2012/697313 |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1572008547 |
Verknüpfungen: | → Zeitschrift |
Potential canonical wnt pathway activation in high-grade astrocytomas / Schüle-Freyer, Rebecca [VerfasserIn]; 2 May 2012 (Online-Ressource)