Active Wnt proteins are secreted on exosomes

• Verfasst von: Groß, Julia Christina [VerfasserIn]
• Verfasst von: Chaudhary, Varun [VerfasserIn]
• Verfasst von: Bartscherer, Kerstin [VerfasserIn]
• Verfasst von: Boutros, Michael [VerfasserIn]
• Titel: Active Wnt proteins are secreted on exosomes
• Verf.angabe: Julia Christina Gross, Varun Chaudhary, Kerstin Bartscherer, and Michael Boutros
• Jahr: 2012
• Umfang: 10 S.
• Fussnoten: Published online 16 September 2012 ; Gesehen am 07.06.2018
• Titel Quelle: Enthalten in: Nature cell biology
• Ort Quelle: New York, NY : Nature America, 1999
• Jahr Quelle: 2012
• Band/Heft Quelle: 14(2012), 10, Seite 1036-1045
• ISSN Quelle: 1476-4679
• DOI: doi:10.1038/ncb2574
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1576139700

Abstract

Wnt signalling has important roles during development and in many diseases. As morphogens, hydrophobic Wnt proteins exert their function over a distance to induce patterning and cell differentiation decisions. Recent studies have identified several factors that are required for the secretion of Wnt proteins; however, how Wnts travel in the extracellular space remains a largely unresolved question. Here we show that Wnts are secreted on exosomes both during Drosophila development and in human cells. We demonstrate that exosomes carry Wnts on their surface to induce Wnt signalling activity in target cells. Together with the cargo receptor Evi/WIs, Wnts are transported through endosomal compartments onto exosomes, a process that requires the R-SNARE Ykt6. Our study demonstrates an evolutionarily conserved functional role of extracellular vesicular transport of Wnt proteins.