BMP-2 and BMP-7 affect human rotator cuff tendon cells in vitro

• Verfasst von: Pauly, Stephan [VerfasserIn]
• Verfasst von: Schmidmaier, Gerhard [VerfasserIn]
• Titel: BMP-2 and BMP-7 affect human rotator cuff tendon cells in vitro
• Verf.angabe: Stephan Pauly, Franka Klatte, Catrin Strobel, Gerhard Schmidmaier, Stefan Greiner, Markus Scheibel, Britt Wildemann
• Jahr: 2012
• Jahr des Originals: 2011
• Umfang: 10 S.
• Fussnoten: Available online 30 March 2011 ; Gesehen am 28.06.2018
• Titel Quelle: Enthalten in: Journal of shoulder and elbow surgery
• Ort Quelle: St. Louis, Mo. : Mosby, 1992
• Jahr Quelle: 2012
• Band/Heft Quelle: 21(2012), 4, Seite 464-473
• ISSN Quelle: 1532-6500
• DOI: doi:10.1016/j.jse.2011.01.015
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: BMP-2
• Sach-SW: BMP-7
• Sach-SW: in vitro
• Sach-SW: Rotator cuff
• Sach-SW: tendon
• Sach-SW: tendon-bone
• Sach-SW: tenocyte
• K10plus-PPN: 1577003055

Abstract

Background Rotator cuff repair is prone to incomplete regeneration. To explore biological improvements of tendon-bone healing, it was the aim of this study to investigate the influence of growth factors bone morphogenetic protein (BMP)-2 and BMP-7 on tenocyte cell activity and matrix gene expression and production. A beneficial effect of these factors would be promising to improve tendon-bone healing in vivo. Methods Tenocyte-like cells were isolated from human rotator cuff tissue samples (supraspinatus and long head of biceps tendon) and incubated with BMP-2 (100-1000 ng/mL) and BMP-7 (100-2000 ng/mL), both alone and in combination. At days 0, 3, and 6, cell activity was assessed. At day 6, collagen type I production and the expression of several tendon-, bone-, and cartilage-related markers (collagen types I-III, osteocalcin, scleraxis) were evaluated. Results Dose-dependent effects of both investigated growth factors on tenocyte-like cells were observed. Application of BMP-2 increased collagen type I production significantly but its expression only slightly. Cell activity was decreased in higher doses over time. For BMP-7, a significant increase in collagen type I production and expression, as well as increased cell activity, was observed. The addition of both factors resulted in decreased parameters when compared with BMP-7 alone. The expression of collagen types II and III, osteocalcin, and scleraxis was not significantly affected by application of BMPs. Conclusion Besides the well-known effects of BMP-2 and BMP-7 on osteoblasts, this study describes further effects on rotator cuff tendon cell biology. Both tissue types potentially need to be addressed to improve tendon-bone healing of the rotator cuff.