Status: Bibliographieeintrag
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| Verfasst von: | Weber, Pascal [VerfasserIn]  |
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| | Batoulis, Helena [VerfasserIn] |
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| | Rink, Kerstin Maren [VerfasserIn] |
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| | Söllner, Thomas [VerfasserIn] |
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| Titel: | Electrostatic anchoring precedes stable membrane attachment of SNAP25/SNAP23 to the plasma membrane |
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| Verf.angabe: | Pascal Weber, Helena Batoulis, Kerstin M Rink, Stefan Dahlhoff, Kerstin Pinkwart, Thomas H Söllner, Thorsten Lang |
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| E-Jahr: | 2017 |
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| Jahr: | March 22, 2017 |
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| Umfang: | 18 S. |
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| Fussnoten: | Gesehen am 22.11.2018 |
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| Titel Quelle: | Enthalten in: eLife |
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| Ort Quelle: | Cambridge : eLife Sciences Publications, 2012 |
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| Jahr Quelle: | 2017 |
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| Band/Heft Quelle: | 6(2017), Artikel-ID e19394 |
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| ISSN Quelle: | 2050-084X |
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| Abstract: | The SNAREs SNAP25 and SNAP23 are proteins that are initially cytosolic after translation, but then become stably attached to the cell membrane through palmitoylation of cysteine residues. For palmitoylation to occur, membrane association is a prerequisite, but it is unclear which motif may increase the affinities of the proteins for the target membrane. In experiments with rat neuroendocrine cells, we find that a few basic amino acids in the cysteine-rich region of SNAP25 and SNAP23 are essential for plasma membrane targeting. Reconstitution of membrane-protein binding in a liposome assay shows that the mechanism involves protein electrostatics between basic amino acid residues and acidic lipids such as phosphoinositides that play a primary role in these interactions. Hence, we identify an electrostatic anchoring mechanism underlying initial plasma membrane contact by SNARE proteins, which subsequently become palmitoylated at the plasma membrane. |
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| DOI: | doi:10.7554/eLife.19394 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: http://dx.doi.org/10.7554/eLife.19394 |
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| | Volltext: https://doi.org/10.7554/eLife.19394 |
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| | DOI: https://doi.org/10.7554/eLife.19394 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | liposome |
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| | membrane targeting |
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| | phosphoinositides |
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| | post-translational modification |
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| | protein electrostatics |
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| | SNAREs |
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| K10plus-PPN: | 1583967613 |
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| Verknüpfungen: | → Zeitschrift |
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Electrostatic anchoring precedes stable membrane attachment of SNAP25/SNAP23 to the plasma membrane / Weber, Pascal [VerfasserIn]; March 22, 2017 (Online-Ressource)
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