Arbuscular cell invasion coincides with extracellular vesicles and membrane tubules

• Verfasst von: Roth, Ronelle [VerfasserIn]
• Verfasst von: Hillmer, Stefan [VerfasserIn]
• Verfasst von: Schumacher, Karin [VerfasserIn]
• Titel: Arbuscular cell invasion coincides with extracellular vesicles and membrane tubules
• Verf.angabe: Ronelle Roth, Stefan Hillmer, Charlotta Funaya, Marco Chiapello, Karin Schumacher, Libera Lo Presti, Regine Kahmann and Uta Paszkowski
• E-Jahr: 2019
• Jahr: 08 February 2019
• Umfang: 8 S.
• Fussnoten: Gesehen am 11.07.2019
• Titel Quelle: Enthalten in: Nature plants
• Ort Quelle: London : Nature Publ. Group, 2015
• Jahr Quelle: 2019
• Band/Heft Quelle: 5(2019), 2, Seite 204-211
• ISSN Quelle: 2055-0278
• DOI: doi:10.1038/s41477-019-0365-4
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1668995220

Abstract

During establishment of arbuscular mycorrhizal symbioses, fungal hyphae invade root cells producing transient tree-like structures, the arbuscules, where exchange of photosynthates for soil minerals occurs. Arbuscule formation and collapse lead to rapid production and degradation of plant and fungal membranes, their spatiotemporal dynamics directly influencing nutrient exchange. We determined the ultra-structural details of both membrane surfaces and the interstitial apoplastic matrix by transmission electron microscopy tomography during growth and senescence of Rhizophagus irregularis arbuscules in rice. Invasive growth of arbuscular hyphae was associated with abundant fungal membrane tubules (memtubs) and plant peri-arbuscular membrane evaginations. Similarly, the phylogenetically distant arbuscular mycorrhizal fungus, Gigaspora rosea, and the fungal maize pathogen, Ustilago maydis, developed memtubs while invading host cells, revealing structural commonalities independent of the mutualistic or parasitic outcome of the interaction. Additionally, extracellular vesicles formed continuously in the peri-arbuscular interface from arbuscule biogenesis to senescence, suggesting an involvement in inter-organismic signal and nutrient exchange throughout the arbuscule lifespan.