| |  Online-Ressource |
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| Verfasst von: | Ahmed, MennatAllah [VerfasserIn]  |
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| | Legrand, Cyril [VerfasserIn] |
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| | Beretta, Carlo Antonio [VerfasserIn] |
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| | Muschko, Alina [VerfasserIn] |
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| | Wegehingel, Sabine [VerfasserIn] |
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| | Müller, Hans-Michael [VerfasserIn] |
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| | Nickel, Walter [VerfasserIn] |
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| Titel: | A time-resolved live cell imaging assay to identify small molecule inhibitors of FGF2 signaling |
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| Verf.angabe: | Mennatallah Ahmed, Cyril Legrand, Ana Yagüe Relimpio, Carlo A. Beretta, Alina Muschko, Sabine Wegehingel, Hans-Michael Müller, Peter Sehr, David W. Will, Joe D. Lewis and Walter Nickel |
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| E-Jahr: | 2019 |
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| Jahr: | 7 June 2019 |
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| Umfang: | 15 S. |
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| Fussnoten: | Gesehen am 17.10.2019 |
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| Titel Quelle: | Enthalten in: Vereinigung der Europäischen Biochemischen GesellschaftenFEBS letters |
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| Ort Quelle: | Chichester : Wiley, 1968 |
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| Jahr Quelle: | 2019 |
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| Band/Heft Quelle: | 593(2019), 16, Seite 2162-2176 |
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| ISSN Quelle: | 1873-3468 |
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| Abstract: | Fibroblast growth factor 2 (FGF2) is a cell survival factor with crucial functions in tumor-induced angiogenesis. Here, we describe a novel time-resolved FGF2 signaling assay based upon live cell imaging of neuroblastoma cells. To validate this system, we tested 8960 small molecules for inhibition of FGF2 signaling with kinetic resolution. Hit compounds were validated in dose-response experiments for FGF2 signaling, FGF receptor antagonism, downstream ERK phosphorylation and FGF2-dependent chemoresistance in a cellular leukemia model system. The new screening system for FGF2 signaling inhibitors has unique features, deselecting compounds with pleiotropic effects on cell proliferation and, along with the experimental pipeline reported, great potential for the discovery of new classes of FGF2 signaling inhibitors that block FGF2 dependent tumor cell survival. |
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| DOI: | doi:10.1002/1873-3468.13462 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1002/1873-3468.13462 |
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| | Verlag: https://febs.onlinelibrary.wiley.com/doi/abs/10.1002/1873-3468.13462 |
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| | DOI: https://doi.org/10.1002/1873-3468.13462 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | FGF2 signaling |
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| | fibroblast growth factor 2 |
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| | live cell imaging |
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| | small molecule inhibitors |
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| | tumor cell survival |
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| | tumor-induced angiogenesis |
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| K10plus-PPN: | 1679058800 |
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| Verknüpfungen: | → Zeitschrift |
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¬A¬ time-resolved live cell imaging assay to identify small molecule inhibitors of FGF2 signaling / Ahmed, MennatAllah [VerfasserIn]; 7 June 2019 (Online-Ressource)
