| |  Online-Ressource |
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| Verfasst von: | Silbernagel, Nicole [VerfasserIn]  |
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| | Balitzki, Jakob [VerfasserIn] |
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| | Hellwig, Andrea [VerfasserIn] |
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| | Hecker, Markus [VerfasserIn] |
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| | Ullrich, Nina D. [VerfasserIn] |
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| Titel: | Shaping the heart |
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| Titelzusatz: | structural and functional maturation of iPSC-cardiomyocytes in 3D-micro-scaffolds |
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| Verf.angabe: | Nicole Silbernagel, Arlene Körner, Jakob Balitzki, Mona Jaggy, Sarah Bertels, Benjamin Richter, Marc Hippler, Andrea Hellwig, Markus Hecker, Martin Bastmeyer, Nina D. Ullrich |
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| Jahr: | 2020 |
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| Jahr des Originals: | 2019 |
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| Umfang: | 12 S. |
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| Fussnoten: | Available online 19 October 2019 ; Gesehen am 13.01.2020 |
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| Titel Quelle: | Enthalten in: Biomaterials |
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| Ort Quelle: | Amsterdam [u.a.] : Elsevier Science, 1980 |
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| Jahr Quelle: | 2020 |
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| Band/Heft Quelle: | 227(2020) Artikel-Nummer 119551, 12 Seiten |
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| ISSN Quelle: | 0142-9612 |
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| Abstract: | Cardiomyocytes derived from induced pluripotent stem cells (iPSC-CMs) represent the best cell source for cardiac regenerative purposes but retain an immature phenotype after differentiation with significant limitations compared to adult cardiomyocytes. Apart from an incomplete cardiomyocyte-specific structure and microarchitecture, cells show at the level of Ca2+ signaling only slow Ca2+ release and reuptake properties. Here, we investigated the effect of restructuring single iPSC-CMs in specially designed 3D-micro-scaffolds on cell morphology and Ca2+ handling. Using direct laser writing, rectangular-shaped scaffolds were produced and single iPSC-CMs were seeded into these forms. Structural analyses revealed strong sarcolemmal remodeling processes and myofilament reorientation in 3D-shaped cells leading to enhanced clustered expression of L-type Ca2+ channels and ryanodine receptors and consequently, to faster Ca2+ transient kinetics. Spontaneous beating activity was enhanced and Ca2+ handling was more robust compared to non-patterned cells. Overall, our data demonstrate for the first time significant improvement of Ca2+ signaling properties in reshaped iPSC-CMs indicative of functional maturation by structural remodeling. |
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| DOI: | doi:10.1016/j.biomaterials.2019.119551 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.biomaterials.2019.119551 |
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| | Verlag: http://www.sciencedirect.com/science/article/pii/S0142961219306507 |
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| | DOI: https://doi.org/10.1016/j.biomaterials.2019.119551 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | 3D-micro-scaffolds |
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| | Ca signaling |
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| | Direct laser writing |
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| | iPSC cardiomyocytes |
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| | Maturation |
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| | Structure-function |
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| K10plus-PPN: | 1687169659 |
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| Verknüpfungen: | → Zeitschrift |
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