Comprehensive DNA methylation analysis of the Aedes aegypti genome

• Verfasst von: Falckenhayn, Cassandra [VerfasserIn]
• Verfasst von: Carneiro, Vitor Coutinho [VerfasserIn]
• Verfasst von: Amarante, Anderson de Mendonça [VerfasserIn]
• Verfasst von: Schmid, Katharina [VerfasserIn]
• Verfasst von: Hanna, Katharina [VerfasserIn]
• Verfasst von: Kang, Seokyoung [VerfasserIn]
• Verfasst von: Helm, Mark [VerfasserIn]
• Verfasst von: Dimopoulos, George [VerfasserIn]
• Verfasst von: Fantappié, Marcelo Rosado [VerfasserIn]
• Verfasst von: Lyko, Frank [VerfasserIn]
• Titel: Comprehensive DNA methylation analysis of the Aedes aegypti genome
• Verf.angabe: Cassandra Falckenhayn, Vitor Coutinho Carneiro, Anderson de Mendonça Amarante, Katharina Schmid, Katharina Hanna, Seokyoung Kang, Mark Helm, George Dimopoulos, Marcelo Rosado Fantappié & Frank Lyko
• E-Jahr: 2016
• Jahr: 02 November 2016
• Fussnoten: Gesehen am 12.05.2020
• Titel Quelle: Enthalten in: Scientific reports
• Ort Quelle: [London] : Macmillan Publishers Limited, part of Springer Nature, 2011
• Jahr Quelle: 2016
• Band/Heft Quelle: 6(2016) Artikel-Nummer 36444, 8 Seiten
• ISSN Quelle: 2045-2322
• DOI: doi:10.1038/srep36444
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1698020961

Abstract

Aedes aegypti mosquitoes are important vectors of viral diseases. Mosquito host factors play key roles in virus control and it has been suggested that dengue virus replication is regulated by Dnmt2-mediated DNA methylation. However, recent studies have shown that Dnmt2 is a tRNA methyltransferase and that Dnmt2-dependent methylomes lack defined DNA methylation patterns, thus necessitating a systematic re-evaluation of the mosquito genome methylation status. We have now searched the Ae. aegypti genome for candidate DNA modification enzymes. This failed to reveal any known (cytosine-5) DNA methyltransferases, but identified homologues for the Dnmt2 tRNA methyltransferase, the Mettl4 (adenine-6) DNA methyltransferase, and the Tet DNA demethylase. All genes were expressed at variable levels throughout mosquito development. Mass spectrometry demonstrated that DNA methylation levels were several orders of magnitude below the levels that are usually detected in organisms with DNA methylation-dependent epigenetic regulation. Furthermore, whole-genome bisulfite sequencing failed to reveal any evidence of defined DNA methylation patterns. These results suggest that the Ae. aegypti genome is unmethylated. Interestingly, additional RNA bisulfite sequencing provided first evidence for Dnmt2-mediated tRNA methylation in mosquitoes. These findings have important implications for understanding the mechanism of Dnmt2-dependent virus regulation.