2D map projections for visualization and quantitative analysis of 3D fluorescence micrographs

• Verfasst von: Sendra, Hernán Gonzalo [VerfasserIn]
• Verfasst von: Hoerth, Christian [VerfasserIn]
• Verfasst von: Wunder, Christian [VerfasserIn]
• Verfasst von: Lorenz, Holger [VerfasserIn]
• Titel: 2D map projections for visualization and quantitative analysis of 3D fluorescence micrographs
• Verf.angabe: G. Hernán Sendra, Christian H. Hoerth, Christian Wunder & Holger Lorenz
• E-Jahr: 2015
• Jahr: 24 July 2015
• Fussnoten: Gesehen am 16.06.2020
• Titel Quelle: Enthalten in: Scientific reports
• Ort Quelle: [London] : Macmillan Publishers Limited, part of Springer Nature, 2011
• Jahr Quelle: 2015
• Band/Heft Quelle: 5(2015) Artikel-Nummer 12457, 8 Seiten
• ISSN Quelle: 2045-2322
• DOI: doi:10.1038/srep12457
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1700664298

Abstract

We introduce Map3-2D, a freely available software to accurately project up to five-dimensional (5D) fluorescence microscopy image data onto full-content 2D maps. Similar to the Earth’s projection onto cartographic maps, Map3-2D unfolds surface information from a stack of images onto a single, structurally connected map. We demonstrate its applicability for visualization and quantitative analyses of spherical and uneven surfaces in fixed and dynamic live samples by using mammalian and yeast cells and giant unilamellar vesicles. Map3-2D software is available at http://www.zmbh.uni-heidelberg.de//Central_Services/Imaging_Facility/Map3-2D.html.