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| Verfasst von: | Dutta, Devanjali [VerfasserIn]  |
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| | Dobson, Adam J. [VerfasserIn] |
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| | Houtz, Philip L. [VerfasserIn] |
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| | Gläßer, Christine [VerfasserIn] |
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| | Revah, Jonathan [VerfasserIn] |
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| | Korzelius, Jerome [VerfasserIn] |
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| | Patel, Parthive H. [VerfasserIn] |
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| | Edgar, Bruce [VerfasserIn] |
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| | Buchon, Nicolas [VerfasserIn] |
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| Titel: | Regional cell-specific transcriptome mapping reveals regulatory complexity in the adult Drosophila midgut |
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| Verf.angabe: | Devanjali Dutta, Adam J. Dobson, Philip L. Houtz, Christine Gläßer, Jonathan Revah, Jerome Korzelius, Parthive H. Patel, Bruce A. Edgar, and Nicolas Buchon |
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| E-Jahr: | 2015 |
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| Jahr: | 2 July 2015 |
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| Umfang: | 13 S. |
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| Fussnoten: | Gesehen am 02.07.2020 |
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| Titel Quelle: | Enthalten in: Cell reports |
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| Ort Quelle: | Maryland Heights, MO : Cell Press, 2012 |
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| Jahr Quelle: | 2015 |
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| Band/Heft Quelle: | 12(2015), 2, Seite 346-358 |
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| ISSN Quelle: | 2211-1247 |
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| Abstract: | Deciphering contributions of specific cell types to organ function is experimentally challenging. The Drosophila midgut is a dynamic organ with five morphologically and functionally distinct regions (R1-R5), each composed of multipotent intestinal stem cells (ISCs), progenitor enteroblasts (EBs), enteroendocrine cells (EEs), enterocytes (ECs), and visceral muscle (VM). To characterize cellular specialization and regional function in this organ, we generated RNA-sequencing transcriptomes of all five cell types isolated by FACS from each of the five regions, R1-R5. In doing so, we identify transcriptional diversities among cell types and document regional differences within each cell type that define further specialization. We validate cell-specific and regional Gal4 drivers; demonstrate roles for transporter Smvt and transcription factors GATAe, Sna, and Ptx1 in global and regional ISC regulation, and study the transcriptional response of midgut cells upon infection. The resulting transcriptome database (http://flygutseq.buchonlab.com) will foster studies of regionalization, homeostasis, immunity, and cell-cell interactions. |
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| DOI: | doi:10.1016/j.celrep.2015.06.009 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1016/j.celrep.2015.06.009 |
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| | Volltext: http://www.sciencedirect.com/science/article/pii/S2211124715006075 |
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| | DOI: https://doi.org/10.1016/j.celrep.2015.06.009 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1703310519 |
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| Verknüpfungen: | → Zeitschrift |
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Regional cell-specific transcriptome mapping reveals regulatory complexity in the adult Drosophila midgut / Dutta, Devanjali [VerfasserIn]; 2 July 2015 (Online-Ressource)
