| |  Online-Ressource |
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| Verfasst von: | Kok, Frédérique [VerfasserIn]  |
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| | Rosenblatt, Marcus [VerfasserIn] |
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| | Teusel, Melissa [VerfasserIn] |
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| | Nizharadze, Tamar [VerfasserIn] |
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| | Gonçalves Magalhães, Vladimir [VerfasserIn] |
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| | Dächert, Christopher [VerfasserIn] |
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| | Maiwald, Tim [VerfasserIn] |
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| | Vlasov, Artyom [VerfasserIn] |
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| | Wäsch, Marvin [VerfasserIn] |
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| | Tyufekchieva, Silvana [VerfasserIn] |
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| | Hoffmann, Katrin [VerfasserIn] |
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| | Damm, Georg [VerfasserIn] |
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| | Seehofer, Daniel [VerfasserIn] |
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| | Böttler, Tobias [VerfasserIn] |
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| | Binder, Marco [VerfasserIn] |
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| | Timmer, Jens [VerfasserIn] |
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| | Schilling, Marcel [VerfasserIn] |
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| | Klingmüller, Ursula [VerfasserIn] |
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| Titel: | Disentangling molecular mechanisms regulating sensitization of interferon alpha signal transduction |
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| Verf.angabe: | Frédérique Kok, Marcus Rosenblatt, Melissa Teusel, Tamar Nizharadze, Vladimir Gonçalves Magalhães, Christopher Dächert, Tim Maiwald, Artyom Vlasov, Marvin Wäsch, Silvana Tyufekchieva, Katrin Hoffmann, Georg Damm, Daniel Seehofer, Tobias Boettler, Marco Binder, Jens Timmer, Marcel Schilling & Ursula Klingmüller |
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| E-Jahr: | 2020 |
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| Jahr: | 21 July 2020 |
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| Umfang: | 32 S. |
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| Fussnoten: | Gesehen am 30.09.2020 |
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| Titel Quelle: | Enthalten in: Molecular systems biology |
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| Ort Quelle: | [London] : Nature Publishing Group UK, 2005 |
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| Jahr Quelle: | 2020 |
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| Band/Heft Quelle: | 16(2020,7) Artikel-Nummer e8955, 32 Seiten |
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| ISSN Quelle: | 1744-4292 |
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| Abstract: | Abstract Tightly interlinked feedback regulators control the dynamics of intracellular responses elicited by the activation of signal transduction pathways. Interferon alpha (IFNα) orchestrates antiviral responses in hepatocytes, yet mechanisms that define pathway sensitization in response to prestimulation with different IFNα doses remained unresolved. We establish, based on quantitative measurements obtained for the hepatoma cell line Huh7.5, an ordinary differential equation model for IFNα signal transduction that comprises the feedback regulators STAT1, STAT2, IRF9, USP18, SOCS1, SOCS3, and IRF2. The model-based analysis shows that, mediated by the signaling proteins STAT2 and IRF9, prestimulation with a low IFNα dose hypersensitizes the pathway. In contrast, prestimulation with a high dose of IFNα leads to a dose-dependent desensitization, mediated by the negative regulators USP18 and SOCS1 that act at the receptor. The analysis of basal protein abundance in primary human hepatocytes reveals high heterogeneity in patient-specific amounts of STAT1, STAT2, IRF9, and USP18. The mathematical modeling approach shows that the basal amount of USP18 determines patient-specific pathway desensitization, while the abundance of STAT2 predicts the patient-specific IFNα signal response. |
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| DOI: | doi:10.15252/msb.20198955 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.15252/msb.20198955 |
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| | Volltext: https://www.embopress.org/doi/full/10.15252/msb.20198955 |
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| | DOI: https://doi.org/10.15252/msb.20198955 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | dynamic pathway modeling |
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| | feedback control |
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| | interferon |
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| | personalized treatment |
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| | signal transduction |
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| K10plus-PPN: | 1734008520 |
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| Verknüpfungen: | → Zeitschrift |
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Disentangling molecular mechanisms regulating sensitization of interferon alpha signal transduction / Kok, Frédérique [VerfasserIn]; 21 July 2020 (Online-Ressource)
