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| Verfasst von: | Roidos, Paris [VerfasserIn]  |
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| | Sungalee, Stéphanie [VerfasserIn] |
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| | Benfatto, Salvatore [VerfasserIn] |
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| | Serçin, Özdemirhan [VerfasserIn] |
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| | Stütz, Adrian M. [VerfasserIn] |
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| | Abdollahi, Amir [VerfasserIn] |
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| | Mauer, Jan [VerfasserIn] |
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| | Zenke, Frank T. [VerfasserIn] |
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| | Korbel, Jan Oliver [VerfasserIn] |
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| | Mardin, Balca R. [VerfasserIn] |
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| Titel: | A scalable CRISPR/Cas9-based fluorescent reporter assay to study DNA double-strand break repair choice |
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| Verf.angabe: | Paris Roidos, Stephanie Sungalee, Salvatore Benfatto, Özdemirhan Serçin, Adrian M. Stütz, Amir Abdollahi, Jan Mauer, Frank T. Zenke, Jan O. Korbel & Balca R. Mardin |
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| E-Jahr: | 2020 |
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| Jahr: | 14 August 2020 |
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| Umfang: | 15 S. |
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| Fussnoten: | Gesehen am 22.10.2020 |
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| Titel Quelle: | Enthalten in: Nature Communications |
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| Ort Quelle: | [London] : Nature Publishing Group UK, 2010 |
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| Jahr Quelle: | 2020 |
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| Band/Heft Quelle: | 11(2020) Artikel-Nummer 4077, 15 Seiten |
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| ISSN Quelle: | 2041-1723 |
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| Abstract: | Double-strand breaks (DSBs) are the most toxic type of DNA lesions. Cells repair these lesions using either end protection- or end resection-coupled mechanisms. To study DSB repair choice, we present the Color Assay Tracing-Repair (CAT-R) to simultaneously quantify DSB repair via end protection and end resection pathways. CAT-R introduces DSBs using CRISPR/Cas9 in a tandem fluorescent reporter, whose repair distinguishes small insertions/deletions from large deletions. We demonstrate CAT-R applications in chemical and genetic screens. First, we evaluate 21 compounds currently in clinical trials which target the DNA damage response. Second, we examine how 417 factors involved in DNA damage response influence the choice between end protection and end resection. Finally, we show that impairing nucleotide excision repair favors error-free repair, providing an alternative way for improving CRISPR/Cas9-based knock-ins. CAT-R is a high-throughput, versatile assay to assess DSB repair choice, which facilitates comprehensive studies of DNA repair and drug efficiency testing. |
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| DOI: | doi:10.1038/s41467-020-17962-3 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1038/s41467-020-17962-3 |
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| | Volltext: https://www.nature.com/articles/s41467-020-17962-3 |
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| | DOI: https://doi.org/10.1038/s41467-020-17962-3 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1736223097 |
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| Verknüpfungen: | → Zeitschrift |
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¬A¬ scalable CRISPR/Cas9-based fluorescent reporter assay to study DNA double-strand break repair choice / Roidos, Paris [VerfasserIn]; 14 August 2020 (Online-Ressource)
