| |  Online-Ressource |
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| Verfasst von: | Paternoga, Helge [VerfasserIn]  |
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| | Früh, Alexander [VerfasserIn] |
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| | Kunze, Ruth [VerfasserIn] |
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| | Bradatsch, Bettina [VerfasserIn] |
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| | Baßler, Jochen [VerfasserIn] |
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| | Hurt, Ed [VerfasserIn] |
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| Titel: | Mutational Analysis of the Nsa2 N-Terminus Reveals Its Essential Role in Ribosomal 60S Subunit Assembly |
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| Verf.angabe: | Helge Paternoga, Alexander Früh, Ruth Kunze, Bettina Bradatsch, Jochen Baßler and Ed Hurt |
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| E-Jahr: | 2020 |
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| Jahr: | 30 November 2020 |
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| Umfang: | 15 S. |
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| Fussnoten: | Gesehen am 18.01.2021 |
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| Titel Quelle: | Enthalten in: International journal of molecular sciences |
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| Ort Quelle: | Basel : Molecular Diversity Preservation International, 2000 |
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| Jahr Quelle: | 2020 |
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| Band/Heft Quelle: | 21(2020,23) Artikel-Nummer 9108, 15 Seiten |
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| ISSN Quelle: | 1422-0067 |
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| | 1661-6596 |
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| Abstract: | The ribosome assembly factor Nsa2 is part of the Rea1-Rsa4-Nsa2 interconnected relay on nuclear pre-60S particles that is essential for 60S ribosome biogenesis. Cryo-EM structures depict Nsa2 docked via its C-terminal β-barrel domain to nuclear pre-60S particles, whereas the extended N-terminus, consisting of three α-helical segments, meanders between various 25S rRNA helices with the extreme N-terminus in close vicinity to the Nog1 GTPase center. Here, we tested whether this unappreciated proximity between Nsa2 and Nog1 is of functional importance. Our findings demonstrate that a conservative mutation, Nsa2 Q3N, abolished cell growth and impaired 60S biogenesis. Subsequent genetic and biochemical analyses verified that the Nsa2 N-terminus is required to target Nsa2 to early pre-60S particles. However, overexpression of the Nsa2 N-terminus abolished cytoplasmic recycling of the Nog1 GTPase, and both Nog1 and the Nsa2-N (1-58) construct, but not the respective Nsa2-N (1-58) Q3N mutant, were found arrested on late cytoplasmic pre-60S particles. These findings point to specific roles of the different Nsa2 domains for 60S ribosome biogenesis. |
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| DOI: | doi:10.3390/ijms21239108 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.3390/ijms21239108 |
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| | Volltext: https://www.mdpi.com/1422-0067/21/23/9108 |
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| | DOI: https://doi.org/10.3390/ijms21239108 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Mdn1 |
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| | Nog1 |
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| | Nsa2 |
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| | Rea1 |
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| | ribosome assembly |
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| | ribosome biogenesis |
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| | Rsa4 |
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| | TINP1 |
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| K10plus-PPN: | 1744881650 |
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| Verknüpfungen: | → Zeitschrift |
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Mutational Analysis of the Nsa2 N-Terminus Reveals Its Essential Role in Ribosomal 60S Subunit Assembly / Paternoga, Helge [VerfasserIn]; 30 November 2020 (Online-Ressource)
