MAP kinase phosphatase 1 is expressed and enhanced by FK506 in surviving mamillary, but not degenerating nigral neurons following axotomy

• Verfasst von: Winter, Christine [VerfasserIn]
• Verfasst von: Schenkel, Johannes [VerfasserIn]
• Verfasst von: Zimmermann, Manfred [VerfasserIn]
• Verfasst von: Herdegen, Thomas [VerfasserIn]
• Titel: MAP kinase phosphatase 1 is expressed and enhanced by FK506 in surviving mamillary, but not degenerating nigral neurons following axotomy
• Verf.angabe: Ch. Winter, J. Schenkel, M. Zimmermann, T. Herdegen
• E-Jahr: 1998
• Jahr: 9 September 1998
• Umfang: 8 S.
• Teil: volume:801
• Teil: year:1998
• Teil: number:1
• Teil: pages:198-205
• Teil: extent:8
• Fussnoten: Gesehen am 06.05.2021
• Titel Quelle: Enthalten in: Brain research
• Ort Quelle: Amsterdam : Elsevier, 1966
• Jahr Quelle: 1998
• Band/Heft Quelle: 801(1998), 1, Seite 198-205
• ISSN Quelle: 1872-6240
• DOI: doi:10.1016/S0006-8993(98)00601-5
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Axotomy
• Sach-SW: FKPB
• Sach-SW: Immunophilin
• Sach-SW: MAP kinase phosphatase
• Sach-SW: Regeneration
• K10plus-PPN: 1757206566

Abstract

The MAP kinase phosphatase 1 (MKP-1), a dual serine-threonine phosphatase, inactivates the MAP kinases ERK and JNK/SAPK which are involved in neuronal survival and neuronal cell death following injury and degenerative stimuli. We have studied by immunocytochemistry whether regulation of MKP-1 is part of the cell-body response following nerve fiber transection. The expression of MKP-1 was investigated in axotomized neurons of the corpus mamillaris (CMm) and substantia nigra pars compacta (SNC) following transection of the mamillo-thalamic tract (MT) and the medial forebrain bundle (MFB), respectively. In contrast to the surviving CMm neurons, the vast majority of SNC neurons undergoes cell death following axotomy. MKP-1 immunoreactivity which is absent in untreated adult rats, appeared in CMm neurons 24 h following MT transection, reached a maximum after 2 days and persisted in a substantial proportion of CMm neurons until 20 days, the end of observation period. In contradistinction, MKP-1 could not be detected in the SNC neurons. MKP-1 immunoreactivity was virtually restricted to the nuclei of neurons. Subcutaneous injection of the immunosuppressant FK506 that protects axotomized SNC neurons against neuronal cell death, enhanced the expression of MKP-1 in CMm, but failed to do so in SNC neurons. The selective expression of MKP-1 in CMm is the first finding on a different regulation of components in the stress kinase signal pathway in surviving vs. degenerating axotomized neurons.