Early delineation of ischemic tissue in rat brain cryosections by high-contrast staining

• Verfasst von: Vogel, Johannes [VerfasserIn]
• Verfasst von: Möbius, Christian [VerfasserIn]
• Verfasst von: Kuschinsky, Wolfgang [VerfasserIn]
• Titel: Early delineation of ischemic tissue in rat brain cryosections by high-contrast staining
• Verf.angabe: Johannes Vogel, Christian Möbius, and Wolfgang Kuschinsky
• E-Jahr: 1999
• Jahr: [May 1999]
• Umfang: 8 S.
• Illustrationen: Illustrationen
• Teil: volume:30
• Teil: year:1999
• Teil: number:5
• Teil: month:05
• Teil: pages:1134-1141
• Teil: extent:8
• Fussnoten: Gesehen am 28.05.2021
• Titel Quelle: Enthalten in: Stroke
• Ort Quelle: New York, NY : Association, 1970
• Jahr Quelle: 1999
• Band/Heft Quelle: 30(1999), 5 vom: Mai, Seite 1134-1141
• ISSN Quelle: 1524-4628
• DOI: doi:10.1161/01.STR.30.5.1134
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1759096709

Abstract

Background and Purpose: After short periods of ischemia, commonly used staining methods yield only moderate differences in optical contrast between normal and damaged brain tissue when gray-scale images are used for computer-assisted image analysis. We describe a high-contrast silver infarct staining (SIS) method that allows an early delineation of ischemic tissue as soon as 2 hours after middle cerebral artery occlusion (MCAO) in rat brain cryosections. Methods: Rats were subjected to permanent MCAO for 2, 4, 6, and 48 hours. The optical densities were quantified in nonischemic white and gray matter and in damaged tissue from gray-scale images of serial sections with the use of a video camera-based image analyzing system. SIS, hematoxylin-eosin, Nissl, and nitroblue tetrazolium stainings were performed in cryosections, and 2,3,5-triphenyltetrazolium hydrochloride (TTC) staining was performed in unfrozen vibratome sections. In addition, the range of reduced cerebral blood flow (CBF) in areas demarcated by SIS was determined in iodo[14C]antipyrine autoradiograms of adjacent cryosections. Results: At all times after MCAO, only SIS showed significantly (P<0.01) lower optical densities in damaged than in normal brain tissue for both white and gray matter. TTC staining was as effective as SIS 6 and 48 hours after MCAO. The tightest correlation between areas of reduced SIS and of reduced CBF was found at a mean ischemic CBF of 22.3 mL/100 g per minute. This corresponds to a CBF range of 0 to 44 mL/100 g per minute in areas of reduced SIS. Conclusions: In contrast to other staining methods, SIS allows a reliable delineation of ischemic brain tissue (core plus penumbra) from nonischemic white and gray matter of rat brain cryosections as soon as 2 hours after MCAO.