Status: Bibliographieeintrag
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| Verfasst von: | Seipp, Stefanie [VerfasserIn]  |
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| | Goeser, Tobias [VerfasserIn] |
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| | Theilmann, Lorenz [VerfasserIn] |
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| | Kallinowski, Birgit [VerfasserIn] |
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| Titel: | Establishment of a highly specific detection system for GB virus C (GBV-C) minus-strand RNA |
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| Verf.angabe: | Stefanie Seipp, Tobias Goeser, Lorenz Theilmann, Birgit Kallinowski |
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| E-Jahr: | 1998 |
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| Jahr: | 18 September 1998 |
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| Umfang: | 7 S. |
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| Teil: | volume:56 |
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| | year:1998 |
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| | number:2 |
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| | month:08 |
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| | pages:183-189 |
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| | extent:7 |
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| Fussnoten: | Gesehen am 24.06.2021 |
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| Titel Quelle: | Enthalten in: Virus research |
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| Ort Quelle: | Amsterdam [u.a.] : Elsevier Science, 1984 |
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| Jahr Quelle: | 1998 |
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| Band/Heft Quelle: | 56(1998), 2 vom: Aug., Seite 183-189 |
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| ISSN Quelle: | 1872-7492 |
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| Abstract: | Although the clinical relevance of GB virus C (GBV-C) is still elusive, this virus has been found with high prevalence in several groups of patients with liver disease. As was shown for hepatitis C virus (HCV), minus-strand RNA is supposed to function as a replicative intermediate. We have established a reliable and sensitive detection system for GBV-C minus-strand RNA based on nested RT-PCR (reverse transcription-polymerase chain reaction) with a tagged primer system. Sensitivity and specificity was extensively tested using in-vitro transcribed GBV-C sequences and genomic viral RNA. Specificity of the amplified fragments was proven by Southern blot hybridization. Using this detection system, we found the presence of GBV-C minus-strand RNA in 6/41 (14.6%) sera of GBV-C infected or GBV-C/HCV coinfected patients. No correlation with virological parameters such as amount of GBV-C plus-strand RNA, genotype or titer of HCV could be detected. |
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| DOI: | doi:10.1016/S0168-1702(98)00071-9 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1016/S0168-1702(98)00071-9 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S0168170298000719 |
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| | DOI: https://doi.org/10.1016/S0168-1702(98)00071-9 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | GBV-C |
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| | Hepatitis virus |
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| | HGV |
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| | Minus-strand RNA detection |
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| K10plus-PPN: | 1761175238 |
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| Verknüpfungen: | → Zeitschrift |
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Establishment of a highly specific detection system for GB virus C (GBV-C) minus-strand RNA / Seipp, Stefanie [VerfasserIn]; 18 September 1998 (Online-Ressource)
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