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Status: Bibliographieeintrag

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Verfasst von:Schmitteckert, Stefanie [VerfasserIn]   i
 Mederer, Tanja [VerfasserIn]   i
 Röth, Ralph [VerfasserIn]   i
 Günther, Patrick [VerfasserIn]   i
 Holland-Cunz, Stefan [VerfasserIn]   i
 Metzger, Marco [VerfasserIn]   i
 Samstag, Yvonne [VerfasserIn]   i
 Schröder-Braunstein, Jutta [VerfasserIn]   i
 Wabnitz, Guido H. [VerfasserIn]   i
 Kurzhals, Stefan [VerfasserIn]   i
 Scheuerer, Jutta [VerfasserIn]   i
 Beretta, Carlo Antonio [VerfasserIn]   i
 Lasitschka, Felix [VerfasserIn]   i
 Rappold, Gudrun [VerfasserIn]   i
 Romero, Philipp [VerfasserIn]   i
 Niesler, Beate [VerfasserIn]   i
Titel:Postnatal human enteric neurospheres show a remarkable molecular complexity
Verf.angabe:Stefanie Schmitteckert, Tanja Mederer, Ralph Röth, Patrick Günther, Stefan Holland-Cunz, Marco Metzger, Yvonne Samstag, Jutta Schröder-Braunstein, Guido Wabnitz, Stefan Kurzhals, Jutta Scheuerer, Carlo A. Beretta, Felix Lasitschka, Gudrun A. Rappold, Philipp Romero, Beate Niesler
E-Jahr:2019
Jahr:18 July 2019
Umfang:14 S.
Fussnoten:Gesehen am 25.06.2021
Titel Quelle:Enthalten in: Neurogastroenterology and motility
Ort Quelle:Oxford [u.a.] : Wiley-Blackwell, 1989
Jahr Quelle:2019
Band/Heft Quelle:31(2019), 10, Artikel-ID 13674, Seite 1-14
ISSN Quelle:1365-2982
Abstract:BACKGROUND: The enteric nervous system (ENS), a complex network of neurons and glial cells, coordinates major gastrointestinal functions. Impaired development or secondary aberrations cause severe enteric neuropathies. Neural crest-derived stem cells as well as enteric neuronal progenitor cells, which form enteric neurospheres, represent a promising tool to unravel molecular pathomechanisms and to develop novel therapy options. However, so far little is known about the detailed cellular composition and the proportional distribution of enteric neurospheres. Comprehensive knowledge will not only be essential for basic research but also for prospective cell replacement therapies to restore or to improve enteric neuronal dysfunction. METHODS: Human enteric neurospheres were generated from three individuals with varying age. For detailed molecular characterization, nCounter target gene expression analyses focusing on stem, progenitor, neuronal, glial, muscular, and epithelial cell markers were performed. Corresponding archived paraffin-embedded individuals' specimens were analyzed accordingly. KEY RESULTS: Our data revealed a remarkable molecular complexity of enteric neurospheres and archived specimens. Amongst the expression of multipotent stem cell, progenitor cell, neuronal, glial, muscle and epithelial cell markers, moderate levels for the pluripotency marker POU5F1 were observed. Furthermore, besides the interindividual variability, we identified highly distinct intraindividual expression profiles. CONCLUSIONS & INFERENCES: Our results emphasize the assessment of molecular signatures to be essential for standardized use, optimization of experimental approaches, and elimination of potential risk factors, as the formation of tumors. Our study pipeline may serve as a blueprint implemented into the characterization procedure of enteric neurospheres for various future applications.
DOI:doi:10.1111/nmo.13674
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.1111/nmo.13674
 Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1111/nmo.13674
 DOI: https://doi.org/10.1111/nmo.13674
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Adolescent
 Cell Culture Techniques
 Child
 enteric nervous system
 Enteric Nervous System
 Epithelial Cells
 Gene Expression Profiling
 human enteric neurospheres
 Humans
 Ileum
 Infant
 Laser Capture Microdissection
 molecular characterization
 Myenteric Plexus
 Myocytes, Smooth Muscle
 nCounter technology
 Neural Crest
 Neural Stem Cells
 Neuroglia
 Neurons
 standardization
 therapeutic potential
 Transcriptome
K10plus-PPN:1761279726
Verknüpfungen:→ Zeitschrift

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