| |  Online-Ressource |
|---|
| Verfasst von: | Schmitt, Sabrina [VerfasserIn]  |
|---|
| | Safferling, Kai [VerfasserIn] |
|---|
| | Westphal, Kathi [VerfasserIn] |
|---|
| | Hrabowski, Manuel [VerfasserIn] |
|---|
| | Müller, Ute [VerfasserIn] |
|---|
| | Angel, Peter [VerfasserIn] |
|---|
| | Wiechert, Lars [VerfasserIn] |
|---|
| | Ehemann, Volker [VerfasserIn] |
|---|
| | Müller, Benedikt [VerfasserIn] |
|---|
| | Holland-Cunz, Stefan [VerfasserIn] |
|---|
| | Stichel, Damian [VerfasserIn] |
|---|
| | Harder, Nathalie [VerfasserIn] |
|---|
| | Rohr, Karl [VerfasserIn] |
|---|
| | Germann, Günter [VerfasserIn] |
|---|
| | Matthäus, Franziska [VerfasserIn] |
|---|
| | Schirmacher, Peter [VerfasserIn] |
|---|
| | Grabe, Niels [VerfasserIn] |
|---|
| | Breuhahn, Kai [VerfasserIn] |
|---|
| Titel: | Stathmin regulates keratinocyte proliferation and migration during cutaneous regeneration |
|---|
| Verf.angabe: | Sabrina Schmitt, Kai Safferling, Kathi Westphal, Manuel Hrabowski, Ute Müller, Peter Angel, Lars Wiechert, Volker Ehemann, Benedikt Müller, Stefan Holland-Cunz, Damian Stichel, Nathalie Harder, Karl Rohr, Günter Germann, Franziska Matthäus, Peter Schirmacher, Niels Grabe, Kai Breuhahn |
|---|
| E-Jahr: | 2013 |
|---|
| Jahr: | September 16, 2013 |
|---|
| Umfang: | 15 S. |
|---|
| Teil: | volume:8 |
|---|
| | year:2013 |
|---|
| | number:9 |
|---|
| | elocationid:e75075 |
|---|
| | pages:1-15 |
|---|
| | extent:15 |
|---|
| Fussnoten: | Gesehen am 08.07.2021 |
|---|
| Titel Quelle: | Enthalten in: PLOS ONE |
|---|
| Ort Quelle: | San Francisco, California, US : PLOS, 2006 |
|---|
| Jahr Quelle: | 2013 |
|---|
| Band/Heft Quelle: | 8(2013), 9, Artikel-ID e75075, Seite 1-15 |
|---|
| ISSN Quelle: | 1932-6203 |
|---|
| Abstract: | Cutaneous regeneration utilizes paracrine feedback mechanisms to fine-tune the regulation of epidermal keratinocyte proliferation and migration. However, it is unknown how fibroblast-derived hepatocyte growth factor (HGF) affects these mutually exclusive processes in distinct cell populations. We here show that HGF stimulates the expression and phosphorylation of the microtubule-destabilizing factor stathmin in primary human keratinocytes. Quantitative single cell- and cell population-based analyses revealed that basal stathmin levels are important for the migratory ability of keratinocytes in vitro; however, its expression is moderately induced in the migration tongue of mouse skin or organotypic multi-layered keratinocyte 3D cultures after full-thickness wounding. In contrast, clearly elevated stathmin expression is detectable in hyperproliferative epidermal areas. In vitro, stathmin silencing significantly reduced keratinocyte proliferation. Automated quantitative and time-resolved analyses in organotypic cocultures demonstrated a high correlation between Stathmin/phospho-Stathmin and Ki67 positivity in epidermal regions with proliferative activity. Thus, activation of stathmin may stimulate keratinocyte proliferation, while basal stathmin levels are sufficient for keratinocyte migration during cutaneous regeneration. |
|---|
| DOI: | doi:10.1371/journal.pone.0075075 |
|---|
| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1371/journal.pone.0075075 |
|---|
| | Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0075075 |
|---|
| | DOI: https://doi.org/10.1371/journal.pone.0075075 |
|---|
| Datenträger: | Online-Ressource |
|---|
| Sprache: | eng |
|---|
| Sach-SW: | Cell cycle and cell division |
|---|
| | Cell migration |
|---|
| | Growth factors |
|---|
| | Keratinocytes |
|---|
| | Mitosis |
|---|
| | Phosphorylation |
|---|
| | Small interfering RNA |
|---|
| | Wound healing |
|---|
| K10plus-PPN: | 1762510804 |
|---|
| Verknüpfungen: | → Zeitschrift |
|---|
Stathmin regulates keratinocyte proliferation and migration during cutaneous regeneration / Schmitt, Sabrina [VerfasserIn]; September 16, 2013 (Online-Ressource)
