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Status: Bibliographieeintrag

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Verfasst von:Remes, Anca [VerfasserIn]   i
 Basha, Dima Ibrahim [VerfasserIn]   i
 Pühler, Thomas [VerfasserIn]   i
 Borowski, Christopher [VerfasserIn]   i
 Hille, Susanne [VerfasserIn]   i
 Kummer, Laura [VerfasserIn]   i
 Wagner, Andreas H. [VerfasserIn]   i
 Hecker, Markus [VerfasserIn]   i
 Soethoff, Jasmin [VerfasserIn]   i
 Lutter, Georg [VerfasserIn]   i
 Frank, Derk [VerfasserIn]   i
 Arif, Rawa [VerfasserIn]   i
 Frey, Norbert [VerfasserIn]   i
 Zaradzki, Marcin [VerfasserIn]   i
 Müller, Oliver J. [VerfasserIn]   i
Titel:Alginate hydrogel polymers enable efficient delivery of a vascular-targeted AAV vector into aortic tissue
Verf.angabe:Anca Remes, Dima Ibrahim Basha, Thomas Puehler, Christopher Borowski, Susanne Hille, Laura Kummer, Andreas H. Wagner, Markus Hecker, Jasmin Soethoff, Georg Lutter, Derk Frank, Rawa Arif, Norbert Frey, Marcin Zaradzki, and Oliver J. Müller
E-Jahr:2021
Jahr:24 February 2021
Umfang:11 S.
Fussnoten:Available online 24 February 2021 ; Gesehen am 26.07.2021
Titel Quelle:Enthalten in: Molecular therapy. Methods & clinical development
Ort Quelle:New York, NY : Nature Publishing Group, 2014
Jahr Quelle:2021
Band/Heft Quelle:21(2021) vom: Juni, Seite 83-93
ISSN Quelle:2329-0501
Abstract:Gene therapeutic approaches to aortic diseases require efficient vectors and delivery systems for transduction of endothelial cells (ECs) and smooth muscle cells (SMCs). Here, we developed a novel strategy to efficiently deliver a previously described vascular-specific adeno-associated viral (AAV) vector to the abdominal aorta by application of alginate hydrogels. To efficiently transduce ECs and SMCs, we used AAV9 vectors with a modified capsid (AAV9SLR) encoding enhanced green fluorescent protein (EGFP), as wild-type AAV vectors do not transduce ECs and SMCs well. AAV9SLR vectors were embedded into a solution containing sodium alginate and polymerized into hydrogels. Gels were surgically implanted around the adventitia of the infrarenal abdominal aorta of adult mice. Three weeks after surgery, an almost complete transduction of both the endothelium and tunica media adjacent to the gel was demonstrated in tissue sections. Hydrogel-mediated delivery resulted in induction of neutralizing antibodies but did not cause inflammatory responses in serum or the aortic wall. To further determine the translational potential, aortic tissue from patients was embedded ex vivo into AAV9SLR-containing hydrogel, and efficient transduction could be confirmed. These findings demonstrate that alginate hydrogel harboring a vascular-targeting AAV9SLR vector allows efficient local transduction of the aortic wall.
DOI:doi:10.1016/j.omtm.2021.02.017
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1016/j.omtm.2021.02.017
 Volltext: https://www.sciencedirect.com/science/article/pii/S2329050121000322
 DOI: https://doi.org/10.1016/j.omtm.2021.02.017
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:endothelial cells
 AAV vector
 adeno-associated virus
 alginate hydrogel
 aorta
 gene therapy
 smooth muscle cells
 vascular disease
 vascular gene transfer
 vascular system
K10plus-PPN:176459407X
Verknüpfungen:→ Zeitschrift

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