| |  Online-Ressource |
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| Verfasst von: | Casadonte, Rita [VerfasserIn]  |
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| | Kriegsmann, Mark [VerfasserIn] |
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| | Kriegsmann, Katharina [VerfasserIn] |
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| | Hauk, Isabella [VerfasserIn] |
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| | Meliß, Rolf R. [VerfasserIn] |
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| | Müller, Cornelia S. L. [VerfasserIn] |
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| | Kriegsmann, Jörg [VerfasserIn] |
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| Titel: | Imaging mass spectrometry-based proteomic analysis to differentiate melanocytic nevi and malignant melanoma |
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| Verf.angabe: | Rita Casadonte, Mark Kriegsmann, Katharina Kriegsmann, Isabella Hauk, Rolf R. Meliß, Cornelia S.L. Müller and Jörg Kriegsmann |
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| E-Jahr: | 2021 |
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| Jahr: | 26 June 2021 |
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| Umfang: | 14 S. |
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| Teil: | volume:13 |
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| | year:2021 |
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| | number:13 |
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| | elocationid:3197 |
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| | pages:1-14 |
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| | extent:14 |
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| Fussnoten: | Gesehen am 18.08.2021 |
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| Titel Quelle: | Enthalten in: Cancers |
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| Ort Quelle: | Basel : MDPI, 2009 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 13(2021), 13, Artikel-ID 3197, Seite 1-14 |
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| ISSN Quelle: | 2072-6694 |
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| Abstract: | The discrimination of malignant melanoma from benign nevi may be difficult in some cases. For this reason, immunohistological and molecular techniques are included in the differential diagnostic toolbox for these lesions. These methods are time consuming when applied subsequently and, in some cases, no definitive diagnosis can be made. We studied both lesions by imaging mass spectrometry (IMS) in a large cohort (n = 203) to determine a different proteomic profile between cutaneous melanomas and melanocytic nevi. Sample preparation and instrument setting were tested to obtain optimal results in term of data quality and reproducibility. A proteomic signature was found by linear discriminant analysis to discern malignant melanoma from benign nevus (n = 113) with an overall accuracy of >98%. The prediction model was tested in an independent set (n = 90) reaching an overall accuracy of 93% in classifying melanoma from nevi. Statistical analysis of the IMS data revealed mass-to-charge ratio (m/z) peaks which varied significantly (Area under the receiver operating characteristic curve > 0.7) between the two tissue types. To our knowledge, this is the largest IMS study of cutaneous melanoma and nevi performed up to now. Our findings clearly show that discrimination of melanocytic nevi from melanoma is possible by IMS. |
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| DOI: | doi:10.3390/cancers13133197 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.3390/cancers13133197 |
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| | Volltext: https://www.mdpi.com/2072-6694/13/13/3197 |
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| | DOI: https://doi.org/10.3390/cancers13133197 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | classification |
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| | imaging mass spectrometry |
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| | MALDI |
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| | melanoma |
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| | nevi |
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| | proteomics |
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| K10plus-PPN: | 1767248911 |
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| Verknüpfungen: | → Zeitschrift |
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Imaging mass spectrometry-based proteomic analysis to differentiate melanocytic nevi and malignant melanoma / Casadonte, Rita [VerfasserIn]; 26 June 2021 (Online-Ressource)
