| |  Online-Ressource |
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| Verfasst von: | Lass, Ulrike [VerfasserIn]  |
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| | Hartmann, Christian [VerfasserIn] |
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| | Capper, David [VerfasserIn] |
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| | Herold-Mende, Christel [VerfasserIn] |
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| | Deimling, Andreas von [VerfasserIn] |
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| | Meiboom, Maren [VerfasserIn] |
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| | Müller, Wolf C. [VerfasserIn] |
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| Titel: | Chromogenic in situ hybridization is a reliable alternative to fluorescence in situ hybridization for diagnostic testing of 1p and 19q loss in paraffin-embedded gliomas |
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| Verf.angabe: | Ulrike Lass, Christian Hartmann, David Capper, Christel Herold-Mende, Andreas von Deimling, Maren Meiboom, Wolf Mueller |
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| Jahr: | 2013 |
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| Umfang: | 8 S. |
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| Teil: | volume:23 |
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| | year:2013 |
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| | number:3 |
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| | month:05 |
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| | pages:311-318 |
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| | extent:8 |
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| Fussnoten: | Published online article accepted 26 October 2012 ; Gesehen am 13.10.2021 |
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| Titel Quelle: | Enthalten in: Brain pathology |
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| Ort Quelle: | Oxford : Wiley-Blackwell, 1990 |
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| Jahr Quelle: | 2013 |
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| Band/Heft Quelle: | 23(2013), 3 vom: Mai, Seite 311-318 |
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| ISSN Quelle: | 1750-3639 |
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| Abstract: | Recent studies imply the importance of rapid and reliable diagnostic assessment of 1p/19q status in oligodendroglial tumors. To date, fluorescence in situ hybridization (FISH) is the most commonly applied technique. FISH, however, has several technical shortcomings that are suboptimal for diagnostic applications: results must be viewed in a fluorescence microscope, results are usually evaluated by a single investigator only, and signal fading excludes physical archiving. Also, in gliomas, the distinction of diffusely infiltrating tumor cells from reactively altered normal tissue may be challenging in fluorescence microscopy. Dual-color chromogenic in situ hybridization (CISH) has started to replace FISH in some diagnostic tests performed in pathology. Here, we present the first single institute experience with a side-by-side analysis of 1p/19q FISH and CISH in a series of 42 consecutive gliomas. FISH and CISH produced identical results for 1p and 19q in 93% of cases (n = 39/42). Discrepant results were reevaluated by repeated FISH and a polymerase chain reaction (PCR)-based microsatellite marker analysis for loss of heterozygosity. Reevaluation confirmed CISH data in all three cases. We conclude that CISH is a reliable alternative in 1p/19q testing in paraffin-embedded tissues likely to be more sensitive to detect 1p/19q status than FISH analysis. |
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| DOI: | doi:10.1111/bpa.12003 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1111/bpa.12003 |
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| | Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1111/bpa.12003 |
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| | DOI: https://doi.org/10.1111/bpa.12003 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | chromogenic in situ hybridization |
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| | chromosome 19q |
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| | chromosome 1p |
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| | fluorescence in situ hybridization |
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| | oligodendroglioma |
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| K10plus-PPN: | 1773521470 |
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| Verknüpfungen: | → Zeitschrift |
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Chromogenic in situ hybridization is a reliable alternative to fluorescence in situ hybridization for diagnostic testing of 1p and 19q loss in paraffin-embedded gliomas / Lass, Ulrike [VerfasserIn]; 2013 (Online-Ressource)
