| |  Online-Ressource |
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| Verfasst von: | Bojcevski, Jovana [VerfasserIn]  |
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| | Wang, Changwen [VerfasserIn] |
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| | Liu, Hai-Kun [VerfasserIn] |
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| | Abdollahi, Amir [VerfasserIn] |
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| | Dokić, Ivana [VerfasserIn] |
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| Titel: | Assessment of normal tissue radiosensitivity by evaluating DNA damage and repair kinetics in human brain organoids |
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| Verf.angabe: | Jovana Bojcevski, Changwen Wang, Haikun Liu, Amir Abdollahi, Ivana Dokic |
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| E-Jahr: | 2021 |
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| Jahr: | 7 December 2021 |
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| Umfang: | 13 S. |
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| Fussnoten: | Gesehen am 19.04.2022 |
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| Titel Quelle: | Enthalten in: International journal of molecular sciences |
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| Ort Quelle: | Basel : Molecular Diversity Preservation International, 2000 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 22(2021), 24, Artikel-ID 13195, Seite 1-13 |
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| ISSN Quelle: | 1422-0067 |
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| | 1661-6596 |
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| Abstract: | DNA-double strand break (DSB), detected by immunostaining of key proteins orchestrating repair, like γH2AX and 53BP1, is well established as a surrogate for tissue radiosensitivity. We hypothesized that the generation of normal brain 3D organoids (“mini-brains”) from human induced pluripotent stem cells (hiPSC) combined with detection of DNA damage repair (DDR) may hold the promise towards developing personalized models for the determination of normal tissue radiosensitivity. In this study, cerebral organoids, an in vitro model that stands in its complexity between 2D cellular system and an organ, have been used. To quantify radiation-induced response, immunofluorescent staining with γH2AX and 53BP1 were applied at early (30 min, initial damage), and late time points (18 and 72 h, residual damage), following clinical standard 2 Gy irradiation. Based on our findings, assessment of DDR kinetics as a surrogate for radiosensitivity in hiPSC derived cerebral organoids is feasible. Further development of mini-brains recapitulating mature adult neuronal tissue and implementation of additional signaling and toxicity surrogates may pave the way towards development of next-generation personalized assessment of radiosensitivity in healthy neuronal tissue. |
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| DOI: | doi:10.3390/ijms222413195 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.3390/ijms222413195 |
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| | kostenfrei: Volltext: https://www.mdpi.com/1422-0067/22/24/13195 |
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| | DOI: https://doi.org/10.3390/ijms222413195 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | brain organoids |
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| | DNA damage repair |
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| | mini-brains |
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| | neurotoxicity |
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| | radiation |
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| | radiosensitivity |
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| K10plus-PPN: | 1799735842 |
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| Verknüpfungen: | → Zeitschrift |
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Assessment of normal tissue radiosensitivity by evaluating DNA damage and repair kinetics in human brain organoids / Bojcevski, Jovana [VerfasserIn]; 7 December 2021 (Online-Ressource)
