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| Verfasst von: | Jamous, Rana M. [VerfasserIn]  |
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| | Boonrod, Kajohn [VerfasserIn] |
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| | Fuellgrabe, Marc W. [VerfasserIn] |
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| | Ali-Shtayeh, Mohammed S. [VerfasserIn] |
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| | Krczal, Gabi [VerfasserIn] |
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| | Wassenegger, Michael [VerfasserIn] |
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| Titel: | The helper component-proteinase of the Zucchini yellow mosaic virus inhibits the Hua Enhancer 1 methyltransferase activity in vitro |
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| Verf.angabe: | Rana M. Jamous, Kajohn Boonrod, Marc W. Fuellgrabe, Mohammed S. Ali-Shtayeh, Gabi Krczal and Michael Wassenegger |
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| E-Jahr: | 2011 |
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| Jahr: | 01 September 2011 |
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| Umfang: | 5 S. |
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| Fussnoten: | Gesehen am 04.07.2022 |
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| Titel Quelle: | Enthalten in: Journal of general virology |
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| Ort Quelle: | Reading : Soc., 1967 |
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| Jahr Quelle: | 2011 |
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| Band/Heft Quelle: | 92(2011), 9, Seite 2222-2226 |
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| ISSN Quelle: | 1465-2099 |
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| Abstract: | The helper component-proteinase (HC-Pro) is a multifunctional protein found among potyviruses. With respect to its silencing suppressor function, small RNA binding appears to be the major activity of HC-Pro. HC-Pro could also exhibit other suppressor activities. HC-Pro may inhibit the Hua Enhancer 1 (HEN1) activity. There is indirect evidence showing that either transient or stable expression of HC-Pro in plants results in an increase of non-methylated small RNAs. Here, we demonstrated that recombinant Zucchini yellow mosaic virus (ZYMV) HC-Pro inhibited the methyltransferase activity of HEN1 in vitro. Moreover, we found that the HC-ProFINK mutant, which has lost small RNA-binding activity, inhibited HEN1 activity, while the truncated proteins and total soluble bacterial proteins did not. Using the ELISA-binding assay, we provided evidence that the HC-ProFRNK wild-type and HC-ProFINK both bound to HEN1, with HC-ProFRNK binding stronger than HC-ProFINK. Motif mapping analysis revealed that the amino acids located between positions 139 and 320 of ZYMV HC-Pro were associated with HEN1 interaction., |
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| DOI: | doi:10.1099/vir.0.031534-0 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1099/vir.0.031534-0 |
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| | Volltext: https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.031534-0 |
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| | DOI: https://doi.org/10.1099/vir.0.031534-0 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1809037786 |
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| Verknüpfungen: | → Zeitschrift |
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¬The¬ helper component-proteinase of the Zucchini yellow mosaic virus inhibits the Hua Enhancer 1 methyltransferase activity in vitro / Jamous, Rana M. [VerfasserIn]; 01 September 2011 (Online-Ressource)
