Nucleolar stress controls mutant Huntington toxicity and monitors Huntington’s disease progression

• Verfasst von: Sönmez, Aynur [VerfasserIn]
• Verfasst von: Mustafa, Rasem [VerfasserIn]
• Verfasst von: Ryll, Salome T. [VerfasserIn]
• Verfasst von: Tuorto, Francesca [VerfasserIn]
• Verfasst von: Wacheul, Ludivine [VerfasserIn]
• Verfasst von: Ponti, Donatella [VerfasserIn]
• Verfasst von: Litke, Christian [VerfasserIn]
• Verfasst von: Hering, Tanja [VerfasserIn]
• Verfasst von: Kojer, Kerstin [VerfasserIn]
• Verfasst von: Koch, Jenniver [VerfasserIn]
• Verfasst von: Pitzer, Claudia [VerfasserIn]
• Verfasst von: Kirsch, Joachim [VerfasserIn]
• Verfasst von: Neueder, Andreas [VerfasserIn]
• Verfasst von: Kreiner, Grzegorz [VerfasserIn]
• Verfasst von: Lafontaine, Denis [VerfasserIn]
• Verfasst von: Orth, Michael [VerfasserIn]
• Verfasst von: Liss, Birgit [VerfasserIn]
• Verfasst von: Parlato, Rosanna [VerfasserIn]
• Titel: Nucleolar stress controls mutant Huntington toxicity and monitors Huntington’s disease progression
• Verf.angabe: Aynur Sönmez, Rasem Mustafa, Salome T. Ryll, Francesca Tuorto, Ludivine Wacheul, Donatella Ponti, Christian Litke, Tanja Hering, Kerstin Kojer, Jenniver Koch, Claudia Pitzer, Joachim Kirsch, Andreas Neueder, Grzegorz Kreiner, Denis L.J. Lafontaine, Michael Orth, Birgit Liss and Rosanna Parlato
• E-Jahr: 2021
• Jahr: 08 December 2021
• Umfang: 12 S.
• Fussnoten: Gesehen am 18.10.2022
• Titel Quelle: Enthalten in: Cell death & disease
• Ort Quelle: London [u.a.] : Nature Publishing Group, 2010
• Jahr Quelle: 2021
• Band/Heft Quelle: 12(2021), 12, Artikel-ID 1139, Seite 1-12
• ISSN Quelle: 2041-4889
• DOI: doi:10.1038/s41419-021-04432-x
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Cell biology
• Sach-SW: Huntington's disease
• K10plus-PPN: 1819124762

Abstract

Transcriptional and cellular-stress surveillance deficits are hallmarks of Huntington’s disease (HD), a fatal autosomal-dominant neurodegenerative disorder caused by a pathological expansion of CAG repeats in the Huntingtin (HTT) gene. The nucleolus, a dynamic nuclear biomolecular condensate and the site of ribosomal RNA (rRNA) transcription, is implicated in the cellular stress response and in protein quality control. While the exact pathomechanisms of HD are still unclear, the impact of nucleolar dysfunction on HD pathophysiology in vivo remains elusive. Here we identified aberrant maturation of rRNA and decreased translational rate in association with human mutant Huntingtin (mHTT) expression. The protein nucleophosmin 1 (NPM1), important for nucleolar integrity and rRNA maturation, loses its prominent nucleolar localization. Genetic disruption of nucleolar integrity in vulnerable striatal neurons of the R6/2 HD mouse model decreases the distribution of mHTT in a disperse state in the nucleus, exacerbating motor deficits. We confirmed NPM1 delocalization in the gradually progressing zQ175 knock-in HD mouse model: in the striatum at a presymptomatic stage and in the skeletal muscle at an early symptomatic stage. In Huntington’s patient skeletal muscle biopsies, we found a selective redistribution of NPM1, similar to that in the zQ175 model. Taken together, our study demonstrates that nucleolar integrity regulates the formation of mHTT inclusions in vivo, and identifies NPM1 as a novel, readily detectable peripheral histopathological marker of HD progression.