| Online-Ressource |
Verfasst von: | Ott, Claus-Eric [VerfasserIn]  |
| Grünhagen, Johannes [VerfasserIn]  |
| Jäger, Marten [VerfasserIn]  |
| Horbelt, Daniel [VerfasserIn]  |
| Schwill, Simon [VerfasserIn]  |
| Kallenbach, Klaus [VerfasserIn]  |
| Guo, Gao [VerfasserIn]  |
| Manke, Thomas [VerfasserIn]  |
| Knaus, Petra [VerfasserIn]  |
| Mundlos, Stefan [VerfasserIn]  |
| Robinson, Peter N. [VerfasserIn]  |
Titel: | MicroRNAs differentially expressed in postnatal aortic development downregulate elastin via 3′ UTR and coding-sequence binding sites |
Verf.angabe: | Claus Eric Ott, Johannes Grünhagen, Marten Jäger, Daniel Horbelt, Simon Schwill, Klaus Kallenbach, Gao Guo, Thomas Manke, Petra Knaus, Stefan Mundlos, Peter N. Robinson |
E-Jahr: | 2011 |
Jahr: | January 31, 2011 |
Umfang: | 12 S. |
Fussnoten: | Gesehen am 19.10.2022 |
Titel Quelle: | Enthalten in: PLOS ONE |
Ort Quelle: | San Francisco, California, US : PLOS, 2006 |
Jahr Quelle: | 2011 |
Band/Heft Quelle: | 6(2011), 1 vom: Jan., Artikel-ID e16250, Seite 1-12 |
ISSN Quelle: | 1932-6203 |
Abstract: | Elastin production is characteristically turned off during the maturation of elastin-rich organs such as the aorta. MicroRNAs (miRNAs) are small regulatory RNAs that down-regulate target mRNAs by binding to miRNA regulatory elements (MREs) typically located in the 3′ UTR. Here we show a striking up-regulation of miR-29 and miR-15 family miRNAs during murine aortic development with commensurate down-regulation of targets including elastin and other extracellular matrix (ECM) genes. There were a total of 14 MREs for miR-29 in the coding sequences (CDS) and 3′ UTR of elastin, which was highly significant, and up to 22 miR-29 MREs were found in the CDS of multiple ECM genes including several collagens. This overrepresentation was conserved throughout mammalian evolution. Luciferase reporter assays showed synergistic effects of miR-29 and miR-15 family miRNAs on 3′ UTR and coding-sequence elastin constructs. Our results demonstrate that multiple miR-29 and miR-15 family MREs are characteristic for some ECM genes and suggest that miR-29 and miR-15 family miRNAs are involved in the down-regulation of elastin in the adult aorta. |
DOI: | doi:10.1371/journal.pone.0016250 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1371/journal.pone.0016250 |
| Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0016250 |
| DOI: https://doi.org/10.1371/journal.pone.0016250 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | Aorta |
| Elastin |
| Gene expression |
| Luciferase |
| Messenger RNA |
| Microarrays |
| MicroRNAs |
| Untranslated regions |
K10plus-PPN: | 1819277666 |
Verknüpfungen: | → Zeitschrift |
MicroRNAs differentially expressed in postnatal aortic development downregulate elastin via 3′ UTR and coding-sequence binding sites / Ott, Claus-Eric [VerfasserIn]; January 31, 2011 (Online-Ressource)