| |  Online-Ressource |
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| Verfasst von: | Ott, Claus-Eric [VerfasserIn]  |
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| | Grünhagen, Johannes [VerfasserIn] |
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| | Jäger, Marten [VerfasserIn] |
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| | Horbelt, Daniel [VerfasserIn] |
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| | Schwill, Simon [VerfasserIn] |
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| | Kallenbach, Klaus [VerfasserIn] |
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| | Guo, Gao [VerfasserIn] |
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| | Manke, Thomas [VerfasserIn] |
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| | Knaus, Petra [VerfasserIn] |
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| | Mundlos, Stefan [VerfasserIn] |
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| | Robinson, Peter N. [VerfasserIn] |
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| Titel: | MicroRNAs differentially expressed in postnatal aortic development downregulate elastin via 3′ UTR and coding-sequence binding sites |
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| Verf.angabe: | Claus Eric Ott, Johannes Grünhagen, Marten Jäger, Daniel Horbelt, Simon Schwill, Klaus Kallenbach, Gao Guo, Thomas Manke, Petra Knaus, Stefan Mundlos, Peter N. Robinson |
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| E-Jahr: | 2011 |
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| Jahr: | January 31, 2011 |
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| Umfang: | 12 S. |
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| Fussnoten: | Gesehen am 19.10.2022 |
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| Titel Quelle: | Enthalten in: PLOS ONE |
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| Ort Quelle: | San Francisco, California, US : PLOS, 2006 |
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| Jahr Quelle: | 2011 |
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| Band/Heft Quelle: | 6(2011), 1 vom: Jan., Artikel-ID e16250, Seite 1-12 |
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| ISSN Quelle: | 1932-6203 |
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| Abstract: | Elastin production is characteristically turned off during the maturation of elastin-rich organs such as the aorta. MicroRNAs (miRNAs) are small regulatory RNAs that down-regulate target mRNAs by binding to miRNA regulatory elements (MREs) typically located in the 3′ UTR. Here we show a striking up-regulation of miR-29 and miR-15 family miRNAs during murine aortic development with commensurate down-regulation of targets including elastin and other extracellular matrix (ECM) genes. There were a total of 14 MREs for miR-29 in the coding sequences (CDS) and 3′ UTR of elastin, which was highly significant, and up to 22 miR-29 MREs were found in the CDS of multiple ECM genes including several collagens. This overrepresentation was conserved throughout mammalian evolution. Luciferase reporter assays showed synergistic effects of miR-29 and miR-15 family miRNAs on 3′ UTR and coding-sequence elastin constructs. Our results demonstrate that multiple miR-29 and miR-15 family MREs are characteristic for some ECM genes and suggest that miR-29 and miR-15 family miRNAs are involved in the down-regulation of elastin in the adult aorta. |
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| DOI: | doi:10.1371/journal.pone.0016250 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1371/journal.pone.0016250 |
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| | Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0016250 |
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| | DOI: https://doi.org/10.1371/journal.pone.0016250 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Aorta |
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| | Elastin |
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| | Gene expression |
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| | Luciferase |
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| | Messenger RNA |
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| | Microarrays |
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| | MicroRNAs |
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| | Untranslated regions |
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| K10plus-PPN: | 1819277666 |
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| Verknüpfungen: | → Zeitschrift |
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MicroRNAs differentially expressed in postnatal aortic development downregulate elastin via 3′ UTR and coding-sequence binding sites / Ott, Claus-Eric [VerfasserIn]; January 31, 2011 (Online-Ressource)
