| |  Online-Ressource |
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| Verfasst von: | Fülöp, Annabelle [VerfasserIn]  |
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| | Marsching, Christian [VerfasserIn] |
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| | Barka, Frederik [VerfasserIn] |
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| | Ucal, Yasemin [VerfasserIn] |
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| | Holfelder, Pauline [VerfasserIn] |
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| | Opitz, Christiane [VerfasserIn] |
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| | Barka, Günes [VerfasserIn] |
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| | Hopf, Carsten [VerfasserIn] |
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| Titel: | Device-controlled microcondensation for spatially confined on-tissue digests in MALDI imaging of N-glycans |
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| Verf.angabe: | Annabelle Fülöp, Christian Marsching, Frederik Barka, Yasemin Ucal, Pauline Pfänder, Christiane A. Opitz, Günes Barka and Carsten Hopf |
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| E-Jahr: | 2022 |
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| Jahr: | 3 November 2022 |
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| Umfang: | 11 S. |
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| Fussnoten: | Gesehen am 11.01.2023 ; This article belongs to the special issue "Mass spectrometry imaging in pharmaceutical research" |
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| Titel Quelle: | Enthalten in: Pharmaceuticals |
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| Ort Quelle: | Basel : MDPI, 2004 |
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| Jahr Quelle: | 2022 |
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| Band/Heft Quelle: | 15(2022), 11, special issue, Artikel-ID 1356, Seite 1-11 |
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| ISSN Quelle: | 1424-8247 |
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| Abstract: | On-tissue enzymatic digestion is a prerequisite for MALDI mass spectrometry imaging (MSI) and spatialomic analysis of tissue proteins and their N-glycan conjugates. Despite the more widely accepted importance of N-glycans as diagnostic and prognostic biomarkers of many diseases and their potential as pharmacodynamic markers, the crucial sample preparation step, namely on-tissue digestion with enzymes like PNGaseF, is currently mainly carried out by specialized laboratories using home-built incubation arrangements, e.g., petri dishes placed in an incubator. Standardized spatially confined enzyme digests, however, require precise control and possible regulation of humidity and temperature, as high humidity increases the risk of analyte dislocation and low humidity compromises enzyme function. Here, a digestion device that controls humidity by cyclic ventilation and heating of the slide holder and the chamber lid was designed to enable controlled micro-condensation on the slide and to stabilize and monitor the digestion process. The device presented here may help with standardization in MSI. Using sagittal mouse brain sections and xenografted human U87 glioblastoma cells in CD1 nu/nu mouse brain, a device-controlled workflow for MALDI MSI of N-glycans was developed. |
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| DOI: | doi:10.3390/ph15111356 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.3390/ph15111356 |
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| | Volltext: https://www.mdpi.com/1424-8247/15/11/1356 |
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| | DOI: https://doi.org/10.3390/ph15111356 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | <i>N</i>-glycans |
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| | enzymatic digestion |
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| | MALDI MSI |
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| | micro-condensation |
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| | standardization |
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| K10plus-PPN: | 183079938X |
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| Verknüpfungen: | → Zeitschrift |
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Device-controlled microcondensation for spatially confined on-tissue digests in MALDI imaging of N-glycans / Fülöp, Annabelle [VerfasserIn]; 3 November 2022 (Online-Ressource)
